摘要
目的:建立准确、灵敏的液相色谱—串联质谱法(LC-MS/MS)测定人血浆中烯丙雌醇的浓度,并评价中国健康女性受试者单剂量口服烯丙雌醇片后的药动学特征。方法:20例女性健康受试者单剂量口服20mg烯丙雌醇片后按时采集血样,以米非司酮为内标,采用APCI正离子选择性反应监测测定烯丙雌醇血浆浓度,计算药动学参数。结果:单剂量口服20mg烯丙雌醇后,烯丙雌醇的主要药动学参数Cmax为(12.48±4.18)ng·ml^(-1),Tmax为(1.9±0.8)h,t1/2为(8.4±3.2)h,MRT0-∞为(6.7±2.6)h,AUC0-36为(41.29±14.83)h·ng·ml^(-1),AUC0∞为(42.72±15.33)h·ng·ml^(-1)。结论:建立的LC-MS/MS测定法结果准确可靠,灵敏度高。
Objective：To estabhsh an accurate and sensitive LC-MS/MS method for the determination of allylestrenol in plasma and to evaluate its pharmacokinetic study in healthy Chinese women volunteers after po the altylestrenol tablets.Methods：The 20 healthy women volunteers received allylestrenot tablet respectively for a single oral dose of allylestrenol 20mg.The plasma concentrations of allylestrenol were determined by a validated LC-APCI-MS/MS method with positive ion SRM mode using mifepristone as internal standard.The pharmaeokinetie parameters were calculated.Results：The main pharmaeokinetic parameters after the single oral dose of allylestrenol tablet（20mg）were as follows：Cmax was（12.48±4.18）ng·ml^-1,Tmax was （1.9±0.8）h,t1/2 was（8.4±3.2）h, MRT0-∞ was（6.7±2.6）h,AUC0-36 was（41.29±14.83）h·ng·ml^-1,AUC0-∞ was （42.72±15.33）h·ng·ml^-1. Conelusions：The validated LC-MS/MS for the determination of allylestrenol in human plasma is accurate,sensitive and reliable.
出处
《中国医药导刊》
2009年第11期1870-1872,共3页
Chinese Journal of Medicinal Guide
