摘要
[目的]调查安徽省某种鸡场发生的以心包炎、肝周炎及腹膜炎为主要症状的疾病的病原,为临床用药提供依据。[方法]采集病料,对病原菌进行分离培养,通过形态学观察、生理生化试验和16SrRNA序列分析对病原菌鉴定,并做药敏试验,根据药敏试验结果进行临床用药治疗。[结果]分离培养得到的病原菌其形态、生理生化特性、16SrRNA序列均与大肠杆菌相符。药敏试验结果显示,该致病菌对菌必治、阿米卡星、壮观霉素等高度敏感;对头孢拉定表现为中度敏感;对呋喃妥因、庆大霉素表现低度敏感。采用菌必治进行治疗,3d后病情得到控制。[结论]对病原菌分离、鉴定及药敏试验是临床上治疗控制鸡大肠杆菌病的有效途径。
[ Objective ] The basis of clinical medicine was provided through the investigation of the pathogen of main symptoms of the diseases such as pericarditis, hepatitis and peritonitis in one chicken farm in Anhui Province, [ Method ] The pathogen of these diseases, cultured after isolated from samples collected, was identified based on the morphological observation, physiological and biochemical tests and 16S rRNA sequence analysis and the clinical drug treatment was conducted according the results of its sensitivity test. [ Results] The cultured pathogen was matched with the E. coli in the morphological, physiological and biochemical characteristics, 16S rRNA sequence. The test result of the drug revealed that the pathogenic bacteria would be highly sensitivity to Jubizhi, amikacin, ADM and others; moderate sensitivity to cefradine and low sensitivity to hitrofurantoin and gentamicin. The disease would be under control in 3 days after the treatment of Jubizhi. [ Conclusion] It was effective way of the clinical treatment of control of chicken E. coli to isolate and identify the pathogen, and to test its sensitivity.
出处
《安徽农业科学》
CAS
北大核心
2009年第33期16382-16383,16388,共3页
Journal of Anhui Agricultural Sciences
基金
江苏省农业三项工程[sx(2008)108]
江苏省教育厅高校高新技术产业发展项目(JHB05-10)
公益性行业(农业)科研专项(子专题)(200803020)
关键词
致病性大肠杆菌
特性
分离鉴定
16
S
RRNA
药敏试验
Escherichia coli
Characteristics
Isolation and identification
16S rRNA
Test of drug-sensitive
