摘要
[目的]探讨ghrelin对蒙古绵羊脐静脉内皮细胞的血管内皮生长因子VEGF及其受体Flt-1mRNA表达的影响。[方法]试验分为4组:I组,空白对照组;II组,脂质体组;III组(SCON),20μmol/L正义寡核苷酸组;IV组(ASCON),20μmol/L反义寡核苷酸组。上述各组均在培养24、36、48h后,采用实时荧光定量方法检测VEGF及其受体Flt-1mRNA表达情况的变化。[结果]VEGFmRNA在I、II组表达量无差异,具有较高的表达量,随着时间的延长无明显变化;在III组VEGFmRNA表达略有降低,但与I和II组间无显著性差异(P>0.05);IV组(反义寡核苷酸组)的VEGFmRNA表达量显著下降(P<0.05),并且随着时间延长逐渐下降。VEGF受体FLT-1mR-NA的表达与VEGF相似。[结论]反义抑制ghrelin对VEGF及其受体Flt-1的mRNA表达有下调作用。
[ Objective ] This study was to investigate the effect of VEGF and its receptor Fh-1 mRNA expression in Mongolia sheep＇ s umbilical vein endothelial cells by ghrelin antisense inhibition. [ Method] Experiments were divided into 4 groups：group Ⅰ （blank control group） ; group Ⅱ （liposome group） ; group Ⅲ（SCON group： 20 μmol/L sense oligonucleotide） ; group Ⅳ （ASCON： 20 μmol/L antisense oligonucleotide）. VEGF and its receptor Fh-1 mRNA expression changes were detected by using real-time fluorescence quantitative detection after 24, 36 and 48 h. [ Result] The expression of VEGF mRNA in group Ⅰ, group Ⅱwere insignificantly different at higher expression levels, and did not change significantly with the time; the expression of VEGF mRNA in group Ⅲ assumed a slight decrease, but there were no significant differences between group Ⅰ and group Ⅱ （P 〉 0.05 ）, the expression of VEGF mRNA in group Ⅳ （ antisense oligonucleotide group ） decreased significantly （ P 〈 0.05 ） ; the expression of VEGF receptor FLT-1 mRNA was similar to that of VEGF [ Conclusion ] Antisense inhibition ghrelin has a downward effect to the expression of VEGF and its receptor Fh-1.
出处
《安徽农业科学》
CAS
北大核心
2009年第33期16277-16279,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30860201)
关键词
生长素
血管内皮生长因子
受体
寡核苷酸类
反义
Ghrelin
Vascular endothelial growth factor
Receptors
Oligonucleotides
Antisense
