摘要
目的:研究表明,黄芪具有一定的促血管生成作用。微乳作为纳米级剂型,近年来在中医药研究领域有着广泛的应用。研究黄芪微乳载入修饰后胶原对大鼠创面毛细血管数及肉芽中血红蛋白和羟脯氨酸含量的影响,进一步探讨黄芪微乳对大鼠血管生成的作用与机制。方法:将50只SD实验大鼠随机分为5组(每组10只):空白胶原组、空白微乳胶原组、血管内皮生长因子(VEGF,250 ng)胶原组、黄芪微乳胶原组和黄芪微乳+VEGF(250 ng)胶原组,各组大鼠分别置入4个相应胶原标本。于造模后第3、7、14 d分3次处死大鼠,取出植入胶原,称取胶原样本质量,分别采用氰化正铁血红蛋白(H ICN)比色法和样本碱水解法,运用公式计算样本血红蛋白和羟脯氨酸含量;照相法观察造模后第14 d处死的大鼠创面颜色,并对大鼠胶原周围组织行病理苏木精-伊红(H-E)染色。结果:在一定的时间,黄芪微乳和黄芪微乳与VEGF联合均能显著增加大鼠肉芽中血红蛋白和羟脯氨酸含量(P<0.05,P<0.01),显著增加大鼠创面毛细血管数量,而在黄芪微乳胶原组与VEGF胶原组比较,差异无统计学意义(P>0.05)。结论:黄芪微乳能增加大鼠毛细血管数量和血红蛋白及羟脯氨酸含量,推测黄芪微乳可能具有促血管生成的作用。
Objective： Astragalus mongholicus could promote angiogenesis.Microemulsion has been widely used in the researches of traditional Chinese medicine in recent years.This study aimed to investigate the effect of Astragalus mongholicus microemulsion modified-collagen on angiogenesis of rats in vivo.Methods： Fifty SD rats were randomly divided into 5 groups： blank collagen,blank microemulsion modified-collagen,vascular endothelial cell growth factor（VEGF） modified-collagen,Astragalus mongholicus microemulsion modified-collagen and Astragalus mongholicus microemulsion plus VEGF modified-collagen.Then four collagen samples were imbedded in each rat.After the establishment of the models,the rats were executed at 3,7 and 14 days.The weights of the collagen samples were measured,and their hematoglobin and hydroxyproline levels were determined by the hemiglobincyanide colorimetric and sampler alkaline hydrolysis methods.The color of the wound surface was observed and the surrounding tissues were examined by HE staining for those killed at 14 days.Results： Both Astragalus mongholicus microemulsion and Astragalus mongholicus microemulsion plus VEGF significantly increased the production of hematoglobin and hydroxyproline（P〈0.05,P〈0.01）,as well as the number of micrangia.However,there were no significant differences between the groups of Astragalus mongholicus microemulsion modified-collagen and VEGF modified-collagen（P〉0.05）.Conclusion： Astragalus mongholicus microemulsion might promote angiogenesis by increasing the number of micrangia and the production of hematoglobin and hydroxyproline.
出处
《医学研究生学报》
CAS
2009年第10期1016-1019,1024,I0001,I0002,共7页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目(批准号:30572332)
关键词
黄芪微乳
胶原
血管内皮细胞生长因子
血管生成
血红蛋白
羟脯氨酸
Astragalus mongholicus microemulsion
Collagen
Vascular endothelial cell growth factor
Angiogenesis
Hematoglobin
Hydroxyproline
