摘要
目的获得含变形链球菌葡糖基转移酶编码基因gtfB的转基因烟草植株。方法将携带变形链球菌葡糖基转移酶编码基因gtfB的植物表达载体p2355-gtfB、p2365-gtfB采用叶盘法经根癌农杆菌介导转化烟草,从而获得抗性烟草植株,并通过GUS染色、npt-Ⅱ基因和部分目的基因片段的PCR扩增对转基因烟草植株进行检测。结果抗性苗经GUS组织染色呈阳性,PCR扩增检测npt-Ⅱ基因及部分目的基因,表明获得了转基因烟草植株。结论目的基因已成功导入烟草基因组中,且具有快速鉴定转化株的优点。
Objective To obtain transgenic tobacco bearing the target gene of glucosytransferase B of Streptococccus mutans. Methods The plant expression vectors of tobacco plant cotyledon were transformed by co-cultivating leaf disks method with Agrobacterium strains harboring the p2355-gtfB and p2365-gtfB plasmids. After selection with antibiotic, the tobacco transformants were analyzed for GUS and total DNA was extracted for PCR amplification npt-Ⅱ and the segment of target gene. Results The tobacco transformants were detected in npt-Ⅱ and the segment of target gene. The results indicated that the transgenic tobacco plants were obtained. Conclusion The target gene was integrated into the tobacco genome successfully. At the same time,the transferred seedling could be selected using quick method.
出处
《口腔医学》
CAS
2009年第10期511-514,共4页
Stomatology
基金
国家自然科学基金资助项目(30160030)
贵州省优秀教育人才省长专项资金(2001年)
关键词
变形链球菌
葡糖基转移酶
植物疫苗
转基因烟草
Streptococccus mutans
glucosytransferaes B
plant vaccine
transgenic tobacco
