摘要
本研究根据形态学和ITS序列分析结果,将一株分离自海南东寨港红树林保护区红海榄根际土壤的菌株XGH2321鉴定为青霉属的一种真菌。通过对培养基中的碳源、氮源和盐度的优化,确定了适合该真菌菌株分泌抑菌活性物质的改良查氏培养基(4%玉米浆,0.3%NaNO3,0.05%KCl,0.1%K2HPO4,0.05%MgSO4,pH 7.4,9%盐度)。将活化后的菌株XGH2321接种到该培养基中,按28°C、160 r/min振荡培养7 d,获得的发酵液水萃取物对金黄色葡萄球菌、藤黄八叠球菌和枯草芽孢杆菌的生长具有明显的抑制作用(MIC为400μg/mL),而发酵液的乙酸乙酯萃取物对上述微生物的生长也具有一定的抑制作用(MIC为800μg/mL);同时,上述2种萃取物对植物病原菌立枯丝核菌的生长也具有明显的抑制作用,MIC分别为200μg/mL和400μg/mL。
The isolate XGH2321, which isolated from the rhizospheric soil of Rhizophora stylosa Griff in Dongzhaigang Mangrove Nature Reserve in China, was identified as a fungus in the genera of Penicillium based on its morphological characteristics and internal transcribed spacer（ITS） sequence analysis. After inoculated in the modified CzapeK-DoX medium（consisted of 4% corn steep, 0.3% NaNO3, 0.05% KCI, 0.1% K2HPO4, 0.05% MgSO4, pH 7.4, 9% salinity）, and cultured under the condition of 28℃ in a rotary shaker at 160 r/min for 7 days, the extracts of ethyl acetate and water-soluble from the fermentation broth showed the apparent antibacterial activities against the growth of Staphylococcus aureus, Sarcina lutea, and Bacillus subtilis with the minimum inhibitory concentration （MIC） of 800 μg/mL and 400 μg/mL, respectively. While at the same time, these two extracts could also suppress the growth of the plant pathogen, Rhizoctonia solani with MIC of 400 μg/mL and 200μg/mL, respectively.
出处
《微生物学通报》
CAS
CSCD
北大核心
2009年第11期1682-1687,共6页
Microbiology China
基金
教育部"新世纪优秀人才支持计划"项目(No.NCET-05-0496)
教育部高等学校科技创新工程重大项目培育资金项目(No.130105)资助
关键词
红海榄
根际土壤
青霉属
抑菌活性
Rhizophora stylosa Griff, Rhizospheric soil, Penicillium, Antibacterial activity
