摘要
对采自连云港海域的海泥样品进行放线菌选择性分离,用其发酵液进行抗肿瘤活性筛选,并对活性较好的菌株HGF26进行了初步鉴定。从海泥样品中共分离得到放线菌78株,以人肝癌细胞HepG2为靶标,活性筛选得到细胞毒活性达60%以上的阳性菌株3株,以其他5株肿瘤细胞为靶标的复筛表明,菌株HGF、26的发酵液对多种肿瘤细胞具有显著的细胞毒活性,其中对胃癌细胞BGC823的细胞毒活性为79%,活性产物具有较好的酸碱和热稳定性。通过对菌株的培养特征、形态特征、生理生化特征和细胞壁成分分析,将菌株HGF26初步鉴定为微白黄链霉菌的海洋变种。
Actinomycete selective isolation was carried out with marine mud samples collected from sea area in Lianynngang, a screening for antitnmor activity with their fermentation broth, and a better strain in the antitumor activity HGF26 was initially characterized. Altogether 78 strains were isolated from the mud samples, 3 positive of them were screened to have over 60% of cytotoxic activity taking human hepatoma cell HepG2 as a target; it was also indicated by re-screening with another five strains tumor cell that fermentation broth of strain HGF26 had a strong cytoxic activity against many kinds of tumor cell, the cytotoxic activity against gastric carcinoma cell BGC823 was as high as 79% , and the active product having acid-and-alkaline and heat stability. It was characterized that HGF26 as Streptomyces albidoflavas var. marinus through culture, morphological, physiological and biochemical features, and cell wall component analysis.
出处
《微生物学杂志》
CAS
CSCD
2009年第5期85-88,共4页
Journal of Microbiology
基金
江苏省海洋资源开发研究院基金项目(JSIMR09A04)
淮海工学院自然科学基金项目(KX07031)
关键词
海洋放线菌
抗肿瘤活性
筛选
菌种鉴定
marine aetinomyeete
antitumor activity
screening
strain characterization
