摘要
The plasmid cDNA library from the liver of Microtus fortis was constructed by using SMART technology. The titers of this cDNA library were 1076 pfu /μL,the recombination rate was about 94%,the library capacity was 1.08×106. 22 cDNAs chosen at random were sequenced. After BLAST analysis of these sequences,it was found that these cDNAs showed more than 75% similarity to the functional known genes of mice,rats and humans in the Genbank. The cDNA library was very important to the research of fatty liver-related gene screening and clones. These provided a base for further study on the molecular biology,fatty liver model development and pathogenesis research of Microtus fortis.
The plasmid cDNA library from the liver of Microtusfortis was constructed by using SMART technology. The titers of this cDNA library were 1076 pfu/μL, the recombination rate was about 94% , the library capacity was 1.08 × 10^6. 22 cDNAs chosen at random were sequenced. After BLAST analysis of these sequences, it was found that these cDNAs showed more than 75% similarity to the functional known genes of mice, rats and humans in the Genbank. The cDNA library was very important to the research of fatty liver-related gene screening and clones. These provided a base for further study on the molecular biology, fatty liver model development and pathogenesis research of Microtus fortis.
出处
《兽类学报》
CAS
CSCD
北大核心
2009年第4期447-450,共4页
Acta Theriologica Sinica
基金
上海市科学基金资助项目(071409001)
