摘要
目的研究NO对肠上皮Caco-2细胞表达分泌片(SC)的影响。方法采用MTT方法观察一氧化氮(NO)对Caco-2细胞的杀伤作用;采用免疫组化、Western blot、real-time PCR方法检测NO对肠上皮细胞表达SC的变化影响。结果高浓度NO对Caco-2细胞有直接杀伤作用。与无NO刺激组比较,用不同浓度NO刺激后SC阳性细胞、Caco-2细胞SC蛋白和SC基因的表达明显减少(P<0.01)。结论NO可直接引起Caco-2细胞损伤,抑制肠上皮细胞分泌SC,因此NO可能削弱肠道免疫防御。
Objective To study the influence of nitric oxide （NO） on the expression of secretory component（SC ） in the intestinal Caco-2 cells.by nitrous oxide （NO）. Methods MTT was used to examine the killing effect of NO on Caco-2 intestinal epithelial cell line. lm- munocytochemistry,Western blot,quantitative real-time PCR were employed to detect SC-positive cells,polymericlg receptor （pLgR）mRNA and protein expression of SC. Results High-dose NO could kill the intestinal epithelial cells directly. SC-positive cell number, SC protein and mRNA expression of NO treated group were less than those of the untreated group （P 〈 0.01 ）. Conclusion NO may impair the intestinal immunity defense through killing the intestinal epithelial cells and inhibiting the expression of secretory component in the intestinal epithelial cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2009年第9期651-653,656,共4页
Journal of China Medical University
基金
国家自然科学基金资助项目(30670947
30871158)
辽宁省教育厅科学研究计划项目(05L466
2008783)
