摘要
目的探讨生存素（survivin）胶体金免疫层析试纸条作为食管癌血清筛选方法的可行性。方法用survivin胶体金免疫层析试纸条检测158例食管癌和146名健康对照者血清标本，并评价其检测性能。结果制备直径20nm的胶体金溶液后，用不同浓度的survivin多抗标记胶体金溶液，确定最佳标记浓度12μg／ml。组装后survivin胶体金免疫层析试纸条检测临床血清标本结果显示，survivin在食管癌患者组中阳性率为51．9％（82／158），在健康对照组阳性率为15．1％（22／146），两组差异有统计学意义（χ^2=45．7，P〈0．05）。survivin阳性率在患者不同年龄组（42～54岁组47．1％，55～68岁组40．9％，69～81岁组63．9％）、不同性别组（男性患者组50．4％，女性患者组58．1％）、不同原发部位组（食管上段组56．3％，中段组46．1％，下段组32．1％）、不同分化程度组（高分化组56．3％，中分化组43．2％，低分化组32．7％）和淋巴结有无转移组（淋巴结转移组43．3％，淋巴结未转移组43．4％）的各组内差异均无统计学意义（χ^2分别为1．11、0．59、2．64、1．63和0．00，P均〉0．05）。survivin胶体金免疫层析试纸条敏感度51．9％（82／158），特异度84．9％（124／146），准确性67．8％（206／304）。结论survivin胶体金免疫层析试纸条操作简单，结果容易判定，可作为食管癌高发人群的筛选方法之一。
Objective To investigate the feasibility of screening for esophageal cancer with survivin colloidal gold immunochromatographic test strip. Methods The serum samples from 158 esophageal cancer patients and 146 healthy individuals were tested with survivin colloidal gold immunochromatographic test strips. Results 20 nm-diameter colloidal gold solution was prepared and survivin polyclonal antibodies with different concentrations were labeled to the gold particles. It showed that the optimal concentration for labeling was 12μg/ml. The prepared survivin colloidal gold immunochromatographic strip test showed positive rates of survivin in esophageal cancer group and control group were 51.9 % （82/158） and 15. 1% （22/146） respectively （χ^2 = 45.7, P 〈 0. 05 ）. No statistical differences were observed in age groups （between 42-54 years： 47. 1% ; between 56-68 years： 40. 9% ; between 69-81years： 63.9% , χ^2= 1.11, P〉0.05）, gender groups （male：50.4% , female：58. 1% ,χ^2=0.59, P 〉0.05） , primary site groups （upper segment： 56. 3% , middle segment： 46. 1% , lower segment： 32. 1% , χ^2 = 2. 64, P 〉 0. 05 ）, differentiation groups （ well-differentiation ： 56. 3 %, moderate differentiation ： 43.2%, poor-differentiation ： 32. 7%, χ^2 = 1.63, P 〉 0. 05 ） and lymph node metastasis groups （ with metastasis： 43.3%, without metastasis ：43.4% , χ^2 = 0. 00, P 〉 0. 05 ）. The sensitivity, specificity and accuracy of survivin colloidal gold immunochromatographic test strip was 51.9% （82/158）, 84. 9% （124/146） and 67. 8% （206/304） respectively. Conclusions Survivin colloidal gold immunochromatographic test strip is fast, simple, easy-to- read. It could be used as a valuable tool for screening of high-risk patients with esophageal cancer.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2009年第10期1143-1147,共5页
Chinese Journal of Laboratory Medicine
关键词
生存素
试纸条
胶体金
免疫层析
食管癌
Survivin
Test strip
Colloidal gold
Immunochromatography
Esophageal cancer
