摘要
目的:探讨丰富环境对快速老化小鼠SAMP8情感的影响.方法:3mo龄SAMP8小鼠和3mo龄SAMR1小鼠随机分为丰富环境组(n=16)和标准环境组(n=10),饲养3mo后用高架十字迷宫实验评测小鼠的情感改变.用RT-PCR方法检测小鼠海马脑源性神经生长因子(BDNF)的表达.结果:丰富环境组小鼠进入开放臂次数比例(OE%)及在开放臂停留的时间(OT%)比例均明显高于标准环境组(P<0.01).丰富环境组海马BDNFmRNA的表达明显高于标准环境组(P<0.01).结论:丰富环境能提高SAMP8小鼠海马BDNFmRNA的表达,并对SAMP8小鼠的精神行为具有干预作用,降低SAMP8小鼠的焦虑行为.
AIM： To explore the effect of enriched environment on emotional behaviour in senescence accelerated-prone mice 8 （ SAMP8 ）. METHODS ： Three-month female SAMP8 and 3-month female senescence accelerated-resistant micel （ SAMR1 ） were divided into Enriched Environment （ EE ） group （ n = 16 ） and Standard Environment（SE） group（n = 10） by random. After 3 months, the emotional behaviour was tested by Elevated Plus-Maze（EPM） and brain derived neurotrophic factor（BDNF） mRNA expression in hippocampus was tested by RT-PCR. RESULTS： EE mice had higher open arm entry percentage （ OE% ） and open arm time percentage（ OT% ） compared with SE mice（P 〈 0. 01）. EE mice had higher BDNF mRNA expression in hippocampus compared with SE mice （P 〈 0.01）. CONCLUSION： EE could improve BDNF mRNA expression in hippocampus of SAMPS. EE also could change the emotional behaviour, which showed that EE could reduce the anxiety behaviour of SAMPS.
出处
《第四军医大学学报》
北大核心
2009年第20期2231-2234,共4页
Journal of the Fourth Military Medical University
基金
河北省自然科学基金(C2009001242)
