摘要
目的:研究电刺激迷走神经对感染性休克心脏功能及超微结构的影响。方法:采用盲肠结扎穿孔法(CLP)复制感染性休克模型。取成年雄性SD大鼠20只,随机分为4组:假CLP组(Sham);CLP组(CLP);迷切组(VGX);电刺激组(STIM)。刺激方法是将左迷走神经远端连接刺激电极,于CLP术毕即刻持续电刺激(5 V、2ms和1 Hz)20 min。各组动物均行颈总动脉置管连续监测平均动脉压(MAP),分别在各组模型制备完毕或者电刺激后0,1,2,4 h检测血浆肿瘤坏死因子α(TNF-α)和4 h抽动脉血行肌酸激酶(CK)、肌酸激酶MB(CK-MB)、乳酸脱氢酶(LDH)水平检测,电镜观察心肌细胞超微结构变化。结果:CLP组和VGX组平均动脉血压进行性下降,血浆TNFα、CK、CK-MB、LDH水平显著升高,透射电镜观察可见脓毒血症大鼠心肌细胞线粒体排列紊乱,空泡变性,肌纤维水肿;与CLP组比较,STIM组动物平均动脉压下降幅度减轻,血浆CK、CK-MB、LDH水平显著降低(P<0.01),电镜心肌损伤减轻。结论:电刺激迷走神经能缓解CLP致感染性休克大鼠的进行性血压下降及炎性因子表达,降低血清CK、CK-MB、LDH水平,减轻心肌组织损害,对心脏功能有潜在的保护作用。
Objective： To investigate the effect of electrical stimulation of efferent vagus on inflammatory response in cardiac function and structure of septic shock rats.Methods： Rat septic shock models were established by cecal ligation and puncture（CLP） method.Twenty male Sprague-Dawley rats were randomly divided into four groups as sham CLP group,CLP group,VGX group（CLP ＋ bilateral cervical vagotomy）,and STIM group（CLP ＋ bilateral cervical vagotomy ＋ electrical stimulation of left vagus nerve trunk）.Mean arterial blood pressure（MAP） was continuously monitored.Rat＇s blood samples were collected at 0,1,2,and 4 h after stimulation and myocardial samples were collected at 4 h in all groups.Serum TNF-α,CK,CK-MB,and LDH were determined.Results： The MAP gradually decreased and the serum TNF-α,CK,CK-MB,and LDH were significantly increased after CLP.Electrical stimulation of the efferent vagus nerve significantly increased MAP,attenuated serum TNF-α,CK,CK-MB,and LDH levels,and alleviated myocardial i...更多nflammation in cardiac tissue at 4 h.Conclusion： Direct electrical stimulation of the peripheral vagus nerve can significantly decrease MAP and attenuate peak serum TNF-α,CK,CK-MB,and LDH levels,alleviate inflammatory response in myocardium,thus it might produce a potential protective effect on myocardium during septic shock in rats.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2009年第5期583-586,共4页
Medical Journal of Wuhan University
基金
国家自然科学基金项目(编号:30672727)
湖北省自然科学基金项目(编号:2004ABA161)
湖北省科技攻关项目(编号:2007AA301B46)
关键词
迷走神经
电刺激
感染性休克
超微结构
Vagus Nerve
Electrial Stimulation
Septic Shock
Ultramicro-Structure
