摘要
目的探讨同种异体骨髓基质细胞(BMSCs)移植到D-氨基半乳糖(D-Gal)诱导的兔急性肝损伤模型的治疗效果。材料与方法BMSCs由雄性白兔的股骨和胫骨的骨髓中提取,分离、培养及传代3周并观察其形态学特征,然后直接肝脏注射移植到D-Gal诱导的同种雄性兔的急性肝损伤模型内。实验组(n=24):为直接肝脏内注射5 ml BMSCs(约2×107个);对照组(n=34):为直接肝脏内注射5 ml D-Hanks液。每天观察各组实验兔的行为变化、存活率、肝功能[丙氨酸氨基转移酶(ALT),谷草转氨酶(AST),白蛋白(ALB)和总胆红素(TBIL)]的改变;BMSCs移植后1、2、4周分别处死两组实验兔,观察肝脏组织病理学变化及肝脏坏死程度,参照评分标准。两位组织病理学医师双盲予以各实验动物的肝脏组织标本评分。结果实验组1周后的存活率高于对照组(95.8%vs70.6%,P<0.05),而1～2周后的存活率较对照组未见显著性改善(100%vs 82.4%,P>0.05)。肝功能的检测显示:与对照组比较,实验组在细胞移植后血清ALB明显升高(P<0.05),ALT和AST均有显著性降低(P<0.05),但两组的TBIL水平的变化并无统计学意义(P>0.05)。组织病理学评分的结果显示:与对照组比较,BMSCs移植到受损肝脏后可显著减轻肝脏坏死程度及刺激肝细胞再生进而发挥强大的抗坏死作用(P<0.001)。结论同种异体BMSCs移植可加速修复D-Gal诱导的兔严重急性肝损伤。BMSCs直接肝内注射移植为实验动物模型简单实用的移植方式。
Objective To investigate the therapeutic effect of infusion of bone marrow stromai cells （BMSCs） on acute hepatic injury （AHI）rabbits induced by D-galactosamine. Materials and Methods BMSCs were derived from bone marrow obtained from femoral and tibial bones of male albino rabbits. BMSCs were separated, grown, and propagated in culture for 3 weeks and were characterized morphologically. They were then infused directly into livers of syngenie male rabbits that received D-galactosamine injection to induce liver injury. AHI rabbits （n = 58） were divided into 2 random groups on the 24th h of DGalN：D-GalN/BMSCs（ n = 24）, to infuse 5 ml （about 2 ×10^7） BMSCs directly as treated group. D-GaIN/D- hanks（ n = 34） ,to infuse the same dose of D-hanks as control. Then behavioral changes of rabbits were monitored everyday. The survival rates were observed and liver functions （ALT, AST, ALB and TBIL）were estimated for all groups. After 1 -4 wk of BMSCs administration, all rabbits were killed with different time （1 wk ,2 wk ,4 wk）respectively and histopathological changes and degrees of necrosis in the hepatic tissues were also assessed. Two histopathologists blinded to treated group and each other＇s results of liver histopathologieal scores. Results Survival rate of D-GalN/BMSCs Group 1 weeks later was higher than that of control （95.8% vs 70.6% ,P 〈0.05）. On the other hand,the survival rate of D-GalN/BM- SCs Group 1 -2 weeks later was not improved significantly as compared with that of control （100% vs 82.4% , P 〉~ 0.05 ）. With regard to liver function, there was also a significant improvement and elevation of serum albumin in the D-GaiN/BM-SCs group compared to the D-GaiN/D-hanks group （ P 〈 0.05 ）. As regard to the liver enzyme ALT and AST, there was also a significant decrease of its level in the D-GaiN/BMSCs group compared to the D-GaiN/D-hanks group （ P 〈 0. 05 ）. However, the level of TBIL of 2 groups was statistically nonsignificant （ P 〉 0. 05 ）. Resul
出处
《临床放射学杂志》
CSCD
北大核心
2009年第10期1459-1464,共6页
Journal of Clinical Radiology
基金
国家自然科学基金项目(编号:30070235
30470508
30870695)
湖南省自然科学基金重点项目(编号:06JJ20081)
湖南省自然科学基金项目(编号:07JJ6040)
关键词
骨髓基质细胞
移植
肝损伤
急性
兔
Bone marrow stromal cells Transplantation Liver injury
acute Rabbit
