摘要
目的:观察外源性精胺(Sp)对传代培养的人脐静脉内皮细胞(HUVECs)的作用,并初步探讨其可能机制。方法:采用传代培养的方法,观察不同浓度精胺(50μmol/L-5mmol/L)对HUVECs作用2h、4h的影响。观察指标:(1)倒置显微镜下观察HUVECs形态学变化;(2)透射电镜观察HUVECs超微结构改变;(3)细胞活力测定;(4)MDA含量测定;(5)SOD活性测定。结果:对HUVECs影响的各项观察指标,50μmol/LSp与正常对照组比较,无显著差异(P>0.05);200μmol/L、1mmol/L、5mmol/LSp对HUVECs有损伤作用,作用4h的细胞损伤较2h重,其特点为细胞活力降低,超微结构损伤,MDA含量升高,SOD活性降低。结论:Sp呈浓度依赖性引起HU-VECs损伤,其作用机制可能与氧自由基大量产生导致的细胞膜脂质过氧化有关。
AIM ： To study the effects of exogenous spermine on human umbilical veins endotheliocytes （ HU- VECs） and to explore its possible mechanism. METHODS ： The serial subculture of HUVECs was used to investigate the effect of exogenous spermine with different concentrations （50 μmol/L-5 mmol/L） on HUVECs in different times （2 h, 4 h）. The morphological changes of HUVECs （by inverted microscope and electron microscope）, the cell viability, the level of MDA and activity of SOD were observed. RESULTS ： Compared to normal control group, no change of all index detected in the group with spermine （50 μmol/L） was observed （P 〉0. 05 ）. Spermine injured HUVECs in a concentration - dependent manner. After adding spermine for 2 h and 4 h, it was observed that cellular injury in 4 h group was more serious than that in 2 h group. The injury of HUVECs caused by exogenous spermine was characterized by decrease in cellular viability and activity of SOD, ultrastructural injury, increase in MDA level. CONCLUSION： Exogenous spermine induces the injury of HUVECs in concentration and time - dependent manners. Its mechanisms may be related to lipid peroxidation induced by increase in the production of oxygen free radical.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第10期1903-1906,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30470688
No.30871012)
教育部高校博士点基金资助项目(No.20070226012)
关键词
精胺
人脐静脉内皮细胞
活性氧
Spermine
Human umbilical vein endothelial cells
Reactive oxygen species
