摘要
利用离子交换及凝胶过滤层析方法对三角帆蚌粗多糖进行分离纯化。试验结果表明:采用弱阴离子交换色谱分离三角帆蚌粗多糖,用0.05mol/LpH7.9的Tris-HCl缓冲液以及0.1、0.2、1mol/LNaCl洗脱液进行分段洗脱,得到4个组分;多糖含量较高的前2个组分经Sepharose 6B凝胶纯化,用双蒸水洗脱到2个组分,分别命名为MPa-1-1和MPa-2-1;经醋酸纤维素薄膜电泳法和高效液相凝胶色谱法鉴定,两者为均一纯多糖,测得MPa-1-1的分子质量为1589.62kDa,MPa-2-1的分子质量为1741.80kDa。
The ion exchange chromatogramphy and gel filtration chromatograhy were utilized to purify mussel polysaccharides in this study. Results showed that mussel coarse polysaccharides could be separated by Marco-Prep DEAE Ion Exchange Media, and gradiantly eluted it with 0.05 mol/L pH7.9 Tris-HCl buffer, 0.1,0.2,1 mol/L NaCl eluant to get 4 components. Then two components with more polysaceharides were purified by Sepharose 6B, and cluted it with pure water to get 2 components named MPa-1-1 and MPa-2-1. By cellulose acetate membrane electrophoresis and HPGPC as purity identification, MPa-1-1 and MPa-2-1 were pure polysaccharides, whose molecular weights were 1 589.62 kDa and 1 741.80kDa respectively.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2009年第5期135-140,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
浙江省科技重点项目(No.2006C2204)
浙江省科技重大攻关项目(No.2006C12067)
关键词
三角帆蚌多糖
纯化
层析
纯度
分子质量
Mussel polysaccharides
purification
chromatography
purity
molecular weight
