摘要
目的:研究急性肝衰竭小鼠肝细胞核DNA(脱氧核糖核酸)的损伤变化及影响因素。方法:健康雌性昆明小鼠腹腔注射D-氨基半乳糖(D-Galn)和脂多糖(LPS)制作急性肝衰竭模型,采用慧星实验检测注药后0.5小时、1小时、2小时、4小时、6小时和8小时小鼠肝细胞核DNA的损伤,ELISA检测血清肿瘤坏死因子-α(TNF-α)浓度。结果:①模型组小鼠于注药0.5小时Olive尾矩值即明显增大,且随时间延伸逐渐增加,与正常组比较,差异均具有显著性意义(P<0.05)。②模型组小鼠于注药0.5小时血清TNF-α浓度无升高;1小时略有升高,但与正常对照组比较,差异无显著性意义;2小时以后,升高趋势明显,各组与正常对照组比较,差异均具有显著性意义。结论:急性肝衰竭小鼠肝细胞核DNA较早出现损伤,并随时间延伸逐渐加重,最终可能诱发了肝细胞凋亡;血清TNF-α浓度的升高与肝细胞核DNA的损伤不完全同步,但其可能具有促进肝细胞DNA损伤的作用。
Objective： To investigate the changes of DNA damage in nuclei of hepatoeytes of mice with acute liver failure and influencing factors. Methods ： Healthy female Kunming white mice were injected intraperitoneally with D-Galactosamine （ D- Gain） and lipopolysaccharide （LPS） to produce the model of acute liver failure, and Comet assay was conducted to test DNA damage in nuclei of hepatocytes of mice 0. 5, 1, 2, 4, 6 and 8 hours after injection , and serum TNF-a level was analyzed by ELISA. Results： （1）The Oliver tail moment of the model group increased significantly 0. 5 hour after injection, and with time extending gradually, the difference between the two groups was significant （P 〈 0. 05 ） . （2The level of serum TNF-α did not increase 0.5 hour after injection, but increased slightly 1 hour after injection. 2 hours later, the increase was significant. Conclusion： The DNA damage occurs in nuclei of hepatocytes of mice with acute liver failure earlier, and with time extending gradually, which may lead to apoptosis of hepatoeytes in the end. The increase level of serum TNF-α is not synchronously with the DNA damage, but it may promote the DNA damage.
出处
《中西医结合肝病杂志》
CAS
2009年第5期295-297,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
