摘要
在酸性介质中,木犀草素在KMnO4-HCHO体系中产生化学发光,据此建立了一种简便、快速测定木犀草素的化学发光新方法。化学发光强度与木犀草素的浓度在5.0×10^-8-1.5×10^-5g/mL范围内呈现出良好的线性关系。其检测限（3σ）为3.0×10^-8g/mL,对8.0×10^-7g/mL的木犀草素溶液进行11次平行测定,相对标准偏差为2.8%。方法可应用于实际样品和合成样品中木犀草素的测定。并探讨了反应的机理。
A flow injection chemiluminescence method for the determination of luteolin, which is based on the enhancement effect of the chemiluminescence produced from the acidic potassium permanganate-formaldehyde system, is described in this paper. Under optimized conditions, the relationship between the enhanced CL intensity and the concentration of luteolin is linear in the ranges of5.0×10^-8-1.5×10^-5g/mL and 3.0×10^-6 - 1.5×10^-5 g/mL with a detection limit （3σ） of 3.0 ×10^-8 g/mL. Relative standard deviation （RSD） is 2.8% （n = 11） for 8.0×10^-7 g/mE luteolin. The proposed method has been successfully applied to the determination of luteolin in real and synthetic samples with satisfactory results. The mechanism of the reaction is also discussed.
出处
《分析试验室》
CAS
CSCD
北大核心
2009年第11期71-73,共3页
Chinese Journal of Analysis Laboratory
