摘要
用冬小麦不同抗旱品种成熟胚的切胚、无菌苗来源的根茎叶切段、幼穗和幼胚等材料为外植体进行愈伤组织的诱导,经大量反复比较试验,结果表明:小麦开花授粉15d左右的幼胚是诱导愈伤组织理想的外植体,不存在基因型差异,不易污染,出愈率高。
Using cut embryo, cut root, stem and leaf, young spike and immature embryo of different drought resistant wheat varieties as explant cultured on modified MS, we found that the immature embryo was the ideal explant to initiate wheat callus. There was no genetype difference and the initiation rate was high but the bacteria-infection rate was very low.
出处
《河北农业科学》
1998年第3期9-12,共4页
Journal of Hebei Agricultural Sciences
