摘要
目的通过对传统试管法与微柱凝胶技术的比较,探讨采用微柱凝胶技术进行新生儿溶血病(HDN)直接抗人球蛋白试验检测的可行性。方法采用试管法与微柱凝胶技术对疑似HDN的1580例黄疸患儿做直接抗人球蛋白试验。结果1580例黄疸患儿试管法直接抗人球蛋白试验阳性652例,其中ABO血型不合618例,Rh血型不合34例;微柱凝胶技术抗人球蛋白试验阳性665例,其中ABO血型不合631例,Rh血型不合34例。两组比较试验结果无显著性差异。将微柱凝胶技术检测结果阳性、试管法阴性的13例做放散试验,阳性12例,阴性1例。结论与传统试管法相比,微柱凝胶技术操作简便、结果易判读,可以提高直接抗人球蛋白试验的阳性率,但也存在一定数量的假阳性。
Objective To investigate the feasibility of micro-column gel immuno-assay （MGIA）for the direct antiglobulin test （DAT） in the hemolytic disease of the newborn （HDN）, and compare MGIA with traditional tube test assay （TTTA）. Methods TTTA and MGIA technique were applied to measure the DAT on 1580 samples suspected with HDN. Results 652 samples were positive for DAT by using TTTA, including 618 cases of ABO HDN and 34 cases of Rh HDN. 665 samples were positive for DAT by using MGIA, including 631 cases of ABO HDN and 34 cases of Rh HDN. There was no significant difference between the two methods （P 〉 0.05）. Conclusions Compared with TTTA, MGIA technique is more feasible and convenient for DAT. MGIA can improve the positive rate of DAT, but has a certain number of false positive.
出处
《北京医学》
CAS
2009年第11期691-692,共2页
Beijing Medical Journal
关键词
新生儿溶血病
直接抗人球蛋白试验
微柱凝胶技术
Hemolytic disease of the newborn （HDN） Direct antiglobulin test （DAT） Micro-colum gel immuno-assay（MGIA）
