摘要
厌氧氨氧化（anaerobic ammonium oxidation,ANAMMOX）菌生长缓慢是影响其实际应用的主要问题之一,选取合适的接种污泥十分重要。本研究采用好氧污泥、厌氧颗粒污泥和厌氧消化污泥3种接种污泥,分别经过61、70和85 d的运行均实现了厌氧氨氧化过程,氨氮去除率分别为82%、92%和91%,总氮去除率达76%、82%和80%。分别接种3种污泥源的厌氧序批式反应器（ASBR）R1、R2、R3出水pH值最终稳定在8.4、8.5、8.5。好氧污泥呈絮状,但沉降性比接种前好,厌氧颗粒污泥解体后最终形成粒径集中在0.5-1.0 mm的污泥,厌氧消化污泥则呈沙化状态,有细小颗粒出现。根据厌氧氨氧化细胞产率系数及NH4^＋-N、NO2--N去除量和NO3--N生成量之间的计量学关系,估算出厌氧氨氧化菌产率系数（以1 mol NH4^＋产生的CH2O0.5N0.15量计）分别为0.080、0.105和0.114 mol.mol^-1,说明反应器内厌氧氨氧化菌有不同程度的衰减。总体而言,厌氧颗粒污泥是富集厌氧氨氧化菌的最适污泥源。
Slow growth of anaerobic ammonium oxidation （ANAMMOX） microbes is one of the main problems with practical application of ANAMMOX process. So it is very important to select a proper type of sludge for inoculation of the ANA- MMOX microbes. Three different types of sludge, i.e. aerobic sludge, anaerobic granular sludge and anaerobic digestion sludge, were selected in the experiment and inoculated with ANAMMOX microbes. After 61,70, 85 days of incubation in anaerobic squencing batch reactors （ASBR） separately, ammonium removal rate reached 82%, 92% , and 91% , respectively and total nitrogen removal rate 76%, 82%, and 80%, respectively in the three samples, and pH of the water discharged from Reactor R1, R2 and R3 remained at 8.4, 8.5 and 8.5, respectively. The aerobic sludge turned into floccules, better in settleability than its precendent and the anaerobic granular sludge was disintegrated into sludge composed of particles of sizes ranging mainly from 0. 5 to 1.0 mm, while the anaerobic digestion sludge became sandy with small particles. Based on the chemometrics of cell yield coefficient of the ANAMMOX bacteria and the NH4^＋-N and NO2^--N removal rate and NO2^--N generation rate , the yield coefficient of the ANAMMOX bacteria were figured out as 0. 080, 0. 105 and 0. 114 mol CH2O0.5N0. 15/mol NH4^＋ , respectively, suggesting that the ANAMMOX bacteria in the reactors attenuated to a varying extent. To sum up, anaerobic granular sludge is the best for enrichment of ANAMMOX bacteria.
出处
《生态与农村环境学报》
CAS
CSSCI
CSCD
北大核心
2009年第4期60-65,共6页
Journal of Ecology and Rural Environment
基金
国家重点基础研究发展规划(2007CB714036)
江苏省自然科学基金(BK2007022)
江苏省太湖水专项(BS2007125)
