摘要
利用RT-PCR方法从拟南芥中分离了1个CK基因家族成员CK1A,该基因的ORF全长2112bp,编码一条703个氨基酸残基的多肽。构建了CK1A基因的植物表达载体35S:GFP:CK1A,采用基因枪法进行的洋葱表皮细胞GFP瞬时表达实验表明,荧光信号主要分布在细胞核上,显示CK1A基因的产物可能在细胞核上发挥作用。半定量RT-PCR分析表明:CK1A基因在花中表达量最大,其次是茎和根,在叶和叶柄中表达量较弱。蓝光使CK1A基因的表达升高,12h时表达量明显增加,24h时表达量下降。酵母双杂交结果显示CK1A蛋白在蓝光下能与CRY2蛋白发生相互作用,暗示CK1A基因可能参与拟南芥的蓝光信号传导途径。
A casein kinase 1 protein gene, CK1A, was isolated from Arabidopsis seedlings by RT-PCR method. This gene contains an open reading frame of 2 112 bp, which encodes 703 amino acids. The plant expression vector of 35S： GFP： CK1A was constructed by the Gateway System. The 35S： GFP： CK1A fusion protein was localized to the nucleus in onion epidermal cell, indicating that the product of CK1A gene plays a role in the cell nucleus. The semi-quantitative RT-PCR analysis showed that CK1A was highly expressed in flowers, stems and roots, but less in leaves and leafstalks. The yeast two-hybrid analysis demonstrated that CK1A and CRY2 can interact in vivo under blue light, which indicates that CK1A may play an important role in blue light signal induction of Arabidopsis.
出处
《遗传》
CAS
CSCD
北大核心
2009年第10期1037-1041,共5页
Hereditas（Beijing)
基金
国家自然科学基金项目(编号:30871325
30600368)资助
