摘要
目的探讨γ-氨基丁酸联合舍曲林对慢性应激抑郁大鼠认知功能及海马神经元形态的影响。方法采用慢性轻度不可预见性应激建立慢性应激抑郁大鼠模型；模型组、舍曲林组、GABA组及研究组大鼠分别给予腹腔注射生理盐水、舍曲林、GABA、GABA＋舍曲林21d，对照组不予任何刺激和药物；采用敞箱试验和电迷宫试验测定大鼠的行为和记忆能力；Nissl染色观察各组大鼠海马CA1、CA3区锥体细胞神经元形态。结果应激后，接受应激的4组的水平运动、垂直运动、修饰运动较对照组相比显著减少（P〈0．05或0．01）。用药后研究组和舍曲林组的水平运动[分别为（57．25±18．79）格，（39．00±13．69）格]、垂直运动[分别为（23．25±7．01）次，（16．75±4．71）次]、修饰运动[分别为（2．25±0．46）次，（2．25±1．04）次]以及电迷宫的正确反应次数[分别为（17．00±1．93）次，（16．88±2．64）次]、潜伏期时间[分别为（172．88±26．91）s，（206．00±33．54）s]均明显好于模型组（P〈0．05或0．01）。GABA组潜伏期时间较模型组显著缩短（P〈0．05）。与对照组相比，舍曲林组、GABA组和研究组的CA1、CA3区锥体细胞神经元在形态、密集度、排列方面均有不同程度的改善，其中研究组的改善最明显。结论GABA联合舍曲林能保护慢性应激抑郁大鼠的海马神经元，从而改善其认知功能.
Objective To investigate the effects of cognitive function and neuron morphous in hippocampus of the chronic stress depressed rats with gamma-aminobutyrie acid and sertraline. Methods Using the chronic mild unpredictable stress to make the model of the chronic stress depressed rats. Sample group, sertraline group, GABA group and research group were gave by saline,sertraline,GABA,GABA and sertraline i.p. respectively for 21 days. Control group recepted no any stress and drugs. Using open-field test and electric maze test to detect the behavior and memory function of the rats. Using Nissl staining to observe the pyramidal neuron morphous in CA1 and CA3 region of the hippocampus. Results After stress,the squares crossing,rears, grooming movement of the four groups accecepted stress were decreased compared with the control（P 〈 0.05）. After treatment, the squares crossing （ respective for 57.25 ± 18.79,39.00 ± 13.69 ）, rears （ respective for 23.25 ± 7.01,16.75 ± 4.71 ）, grooming movement（ respective for 2.25 ± 0.46,2.25 ± 1.04）, number of correct reaction （ respective for 17.00 ± 1.93,16.88 ± 2.64 ） and latency time（ respective for 172.88 ±26.91,206. 013 ± 33.54 ） of the research group and sertralinc group were better than sample group（P 〈 0.05 or 0.01 ）. GABA group showed no significant improvement except for the reduced latency time compared with the sample group（P〈0, 05）. Compared with the control, the morphous, numbers and alignment of pyramidal neuron in CA1 and CA3 in hippocampus of the sertraline group, GABA group and research group were improve with different level,all the better in research group. Conclusion GABA with sertraline can improve the cognitive function of the chronic stress depressed rats by protect the neuron in hippocampus.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2009年第10期912-914,共3页
Chinese Journal of Behavioral Medicine and Brain Science
关键词
慢性应激
抑郁
Γ-氨基丁酸
海马
认知
Chronic stress
Depression
Gamma-aminobutyric acid
Hippocampus
Cognition
