摘要
目的探讨一种可行的方法对抗CCL4腹腔注射诱导的大鼠肝纤维化模型的自然恢复趋势,以确保药物或细胞移植等方法对肝纤维化治疗效果的可靠性。方法50%CCL4腹腔注射诱导大鼠肝纤维化模型,然后随机分4组改皮下注射不同剂量CCL4（0.02-0.08ml/100g）,通过纤维化程度分期、纤维化半定量统计分析、肝功能和血清肝纤维化指标测定来评估肝纤维化。结果CCL4腹腔注射6周,肝组织汇管区和血管周围出现较粗大的纤维间隔。在抗逆转实验的第2周和第4周,皮下注射0.06ml/100g体重CCL4组的纤维化水平与造模成功时的差异无统计学意义（P〉0.05）。结论对大鼠肝纤维化模型使用皮下注射50%CCL4,0.06ml/100g体重,1次/周,能够将造模时的纤维化水平维持4周。
Obejctive For a viable method to oppose the natural recovery trend of CCL4-induced liver fibrosis model in rats by intraperitoneal injection after stopping induction.To ensure that drugs,cell transplantation and other methods of treatment to liver fibrosis isreliable.Methods 50%CCL4 intraperitoneal injection,twice a week,sustaining six weeks induced liver fibrosis model in rats,Then all rats are divided into 4 groups randomly and subcutaneous injected with different doses 50% CCL4（0.02～0.08ml/100g weight）,once a week,continuing four weeks,through the liver index,fibrosis stage,fibrosis semi-quantitative statistical analysis,liver function and serum index of liver fibrosis to assess liver fibrosis.Results CCL4 intraperitoneal injection six weeks,the header district of liver tissue and blood vessels appear coarse fibers interval.In anti-reversal experimentation＇s first two weeks and four weeks,the statistical difference of the level between fibrosis model and subcutaneous injection of 0.06ml/100g weight CCL4 group was not significant（P〈0.05）.Conclusion To a successful rat liver fibrosis model,stopping intraperitoned and altering subcutaneous injection of 50% CCL4 0.06ml/100g weight,once a week,could be able to maintain the level of fibrosis four weeks.
出处
《四川医学》
CAS
2009年第10期1513-1515,共3页
Sichuan Medical Journal
基金
四川省教育厅资助课题(编号:2003-A027)
关键词
对抗
大鼠
肝纤维化
自发逆转
to oppose
rats
liver fibrosis
natural recovery
