摘要
应用美国AmpFISTR Indentifiler荧光标记复合扩增试剂盒，结合PE9700型PCR仪和美国ABI公司310型遗传分析仪，对湖南汉族人群D8S1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818和FGA共15个STR基因座进行多态性调查分析．结果显示15个STR基因座的基因型分布符合Hardy．Weinberg平衡。其杂合度（H）介于0．593～0．900，多态信息含量（PIC）介于O．54～0．85，个体识别力（DP）介于0．780～0．963，非父排除率（PE）介于0．282～0．785，累计个体识别力为（1～1．6×10^-17）〉0．99999999。累计非父排除率为0．9999995．证明15个STR基因座在湖南省汉族人群中具有较高的多态性。可应用于该地区群体学研究、法医学个体识别和亲权鉴定等．
The polymorphism distributions of 15 STR loci （D8Sl179, D21Sll, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA） were investigated in a Hunan Han population using AmpF1STR indentifiler kit, GeneAmp PCR system 9700 and ABI 310 genetic analyzer. Gene frequency, heterozygosity （H）, polymorphism information content （PIC）, power of discrimination （DP） and probability of paternity exclusion （PE） were calculated, and all loci were tested for Hardy-Weinberg equilibrium. Results indicate that the gene frequency of these 15 STR loci is in Hardy-Weinberg equilibrium. The H is at 0.593-0,900, PIC is at 0.54-0.85, DP is at 0.780-0.963, PE is at 0.282-0.785. Cumulative DP of the 15 STR is （1-1.6×10^-17） 〉 0.999 999 99, and the cumulative PE is 0.999 999 5. Therefore, the 15 STR loci used in this study are highly polymorphic in Hunan Han population and can be applied to population study, individual identification and paternity testing in forensic science.
出处
《生命科学研究》
CAS
CSCD
2009年第5期399-402,421,共5页
Life Science Research
关键词
短串联重复序列
遗传多态性
法医学
short tandem repeat
genetic polymorphism
forensic science
