摘要
以160头荷斯坦和娟姗奶牛作为研究对象,分别测定了其在不同温度条件下,直肠温度、呼吸频率、产奶量及乳成分,并进行了统计分析。同时设计引物扩增HSP70基因3'-侧翼区,通过PCR-SSCP技术分析HSP70基因3'-侧翼区的多态性,并发现一个多态位点。结果表明:扩增片段为292bp,扩增产物有基因多态性,共发现4种基因型,分别为荷斯坦牛的AA(H)型、AB(H)型和娟姗牛的AA(J)型、AB(J)型。其中B基因可能为抗热应激基因,这4种基因型奶牛个体的生产性能差异不显著(P>0.05)。
One hundred and sixty Holstein and Jersey cows were used to compare the rectal temperature, respiration rate, milk yield and milk components at different temperature environment. The single-strand conformation polymorphism （PCR-SSCP） method was used to analyze the polymorphisru in 3 ＇ -flanking region of the HSP70 gene. As results show： The PCR-SSCP products of primer had 292 hp and showed polymorphisms. Four genotypes were found and could be divided into AA, AB in Holstein cows and AA, AB in Jersey cows. Among them, B gene may be the resistance heatshock gene. The production traits of these four genotypic cows are not significant （ P 〉 0. 05 ）.
出处
《激光生物学报》
CAS
CSCD
2009年第5期614-618,共5页
Acta Laser Biology Sinica
基金
"十一五"国家科技支撑计划项目(2006BAD04A01
2006BAD04A12)
芜湖市科技计划重点项目(2008620)
安徽省教育厅项目(KJ2009A039)
关键词
直肠温度
HSP70基因
多态性
热应激
rectal temperature
HSP70gene
polymorphisms
heat-shoek stress
