摘要
目的:探讨卵清蛋白免疫耐受因子(OVA immune tolerance factors,OVAITFs)转移抗原特异性外周免疫耐受的特性。方法:分别从OVA耐受鼠或空白小鼠的脾淋巴细胞裂解液中分离分子量小于3kD的组分,即为OVA ITFs或OVA ITFs control。试验BALB/c小鼠分成5组,A、B、C、D组经尾静脉分别注射OVA ITFs、OVA ITFs control、OVA耐受鼠脾淋巴细胞或PBS给正常BALB/c小鼠,E组不做任何处理。流式细胞术检测转移前后受体鼠脾脏CD4+CD25+ T细胞亚群的比例变化,MTT法检测受体鼠OVA特异性T淋巴细胞的增殖情况,夹心ELISA法测定培养上清中IL-10、TGF-β1的水平。结果:转移OVA ITFs或OVA耐受鼠脾淋巴细胞后,受体鼠脾脏CD4+CD25+ T细胞亚群在总CD4+ T细胞的比例分别为(15.32±1.03)%和(15.35±0.62)%,与转移前(9.97±1.38)%相比显著上升(P<0.05);OVA特异性淋巴细胞增殖刺激指数(Stimulation Index,SI)分别为0.699±0.05和0.704±0.03,与PBS对照组(1.356±0.07)相比明显受到抑制(P<0.05);培养上清中TGF-β1的分泌量分别为129.15±6.14和106.71±2.89pg/ml,显著高于PBS对照组(52.82±3.68pg/ml,P<0.01),IL-10分泌量未检出。转移OVA ITFs control后,受体小鼠的CD4+CD25+ T细胞比例、OVA特异性淋巴细胞增殖及TGF-β1、IL-10分泌量均无显著变化。结论:OVA ITFs具有将OVA特异性免疫耐受传递给正常受体小鼠的特性。
Objective：To investigate the immunoregulation effect of ovalbumin immune tolerance factors （OVA ITFs）. Methods： Components that were smaller than 3 kD were isolated from the splenic lymphocytes lysates of OVA tolerance mice or naive mice, respectively, named as OVA ITFs or OVA ITFs conttrol. Naive BALB/c mice were divided into 5 groups, group A, B, C, D were injected i. v. by OVA ITFs, OVA ITFs control, splenic lymphocytes from OVA tolerant mice or PBS, respectively, group E as the blank control. The percentages of CD^＋ CD25^＋ T cell subpopulation from spleens before and after adoptive transfer were measured with flow cytometer; OVA specific lymphocyte responses were assessed by MTF assay. The levels of IL- 10 and TGF-β1 in the cuhure supematants were tested by ELISA kits. Results： For OVA ITFs or splenic lymphocytes from OVA tolerant mice, the percentages of CD4^＋ CD25^＋ T cell subpopulation from spleens after adoptive transfer were raised significantly compared with that before adoptive transfer （ 15.32 %±1.03 % and 15.35 %± 0.62 % vs 9.97 % ± 1.38 %, P 〈 0. 05）. The stimulation index of OVA-specific lymphocytes responses were 0. 699 ± 0.05 and 0. 704 ±0.03 respectively, lymphocytes proliferation were suppressed significantly when compared with that of PBS control group（ 1. 356 ± 0.07, P 〈 0.05 ）. The production of active TGF-β1 was showed of profound increases compared with that of PBS control group （ 129.15 ± 6.14 and 106.71± 2.89 pg/ml vs 52.82 ± 3.68 pg/ml, P 〈 0.01 ）, but IL-10 levels were not detectable. For OVA ITFs control, no significant differences were observed before and after adoptive transfer on either percentage of CD4^＋ CD25^＋ T cell subpopulation, responses of OVA specific lymphocytes or IL-10 and TGF-β1 levels. Conclusion： OVA 1TFs can transfer OVA specific immune tolerance to naive recipient mice.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2009年第10期890-894,共5页
Chinese Journal of Immunology
基金
中国博士后科学基金(20070420219)资助项目
