摘要
目的:建立以高效液相色谱法测定一清颗粒中大黄酚含量的方法。方法:Thermo-HypersilC18(250mm×4.6mm,5μm),流动相:甲醇-0.1%磷酸溶液(65∶35),流速:1.0mL·min-1,检测波长:254nm,柱温:40℃。结果:大黄酚在0.0102～0.2042μg呈良好的线性关系。结论:本方法简便、准确、分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制。
Objective： To establish HPLC method for the determination of chrysophanol in Yiqing Particles. Methods： Thermo-HypersilC 18 （ 250mm × 4.6mm,5μm）, mobile phase was methanol-0. 1% phosphoric acid （65：35） with flow rate at 0. 8mL.min^-1, and detection wavelength at 254nm, column temperature was 40℃. Results： Chrysophanol had good linear relation in the range of 0. 010 2 -0. 204 2μg. Conclusion： The method is simple, accurate, good separation efficiency, wide linear range and high sensitivity. The method can be used to control the quality of Yiqing Particles.
出处
《中国现代中药》
CAS
2009年第10期26-27,40,共3页
Modern Chinese Medicine
