摘要
目的通过分析浙江8例间日疟本地病例的分子流行病学特征,探讨MSP-1基因在间日疟流行病学调查中的意义,为制定疟疾防制策略提供科学依据。方法用套氏PCR方法对间日疟本地病例血样扩增MSP-1基因片段,测定DNA序列,并用Clustal X、GeneDoc、MEGE 4.1等软件进行比对、作遗传图谱等分析。结果8例经镜检确诊为间日疟病例的MSP-1基因序列分为Sal-1型和重组Ⅲ型2种:前者包括安吉3例;后者包括建德4例、义乌1例。结论在结合较为详细的个案调查资料情况下,MSP-1基因可作为分子流行病学的标志物,对间日疟进行基因分型和判断感染来源。
Objective To explore the epidemiological significance of MSP-1 in vivax malaria by analyzing the molecular epidemiological characteristics of local malaria infectors in Zhejiang province and provide scientific evidence for malaria control strategies. Methods The fragment of MSP-1 gene from blood samples of local infectors with vivax malaria was amplified and the DNA sequence was determined by nested PCR. The software such as Clustal X, GeneDoc, MEGE 4.1 were used for comparing and making genetic map. Results The MSP-1 gene sequence of 9 cases with vivax malaria diagnosed by microscopic examination were identificated as Sal-1 type and recombinant type Ⅲ. The first type included three local cases of Anji. The second type included four local cases of Jiande, one imported case of Anji as well as one local case of Yiwu. Conclusion Combining with more detailed information from individual investigation, MSP-1 gcne can be used as a molecular epidemiological marker to genotyping plasmedium vivax and to determine the source of infection.
出处
《浙江预防医学》
2009年第10期1-3,共3页
Zhejiang Journal of Preventive Medicine
关键词
间日疟原虫
MSP-1基因
本地疟疾
Plasmodium vivax
Merozoite Surface Protein 1 （PvMSP-1）
Local malaria
