摘要
目的探寻导致蛋白印迹法(western blotting,WB)检测结果呈"HIV抗体不确定"可能的生物学因素。方法检测经WB确证后结果为"HIV抗体不确定"病例乙型肝炎(type B hepatitis,HBV)、丙型肝炎(type Chepatitis,HCV)、梅毒螺旋体(treponemiasis,TP)、HTLV-I/II(human T cell leukemia virus I/II,HTLV-I/II)的感染状况及癌胚抗原(carcino-embryonic antigen,CEA)、抗核抗体(antinuclear antibody,ANA)、甲胎蛋白(α-fetoprotein,AFP)、β2微球蛋白(β2-microglobulin,β2-MG)在血液中的含量,并与文献资料报道的普通人群/健康人比较。结果"HIV抗体不确定"病例HCV抗体初筛阳性率、TP抗体阳性率和HTLV-I/II抗体的初筛阳性率均高于普通人群;22.22%的"HIV抗体不确定"病例血液中ANA含量高于普通人群,呈阳性或可疑;9.27%的"HIV抗体不确定"非孕产妇病例血液中AFP含量高于普通人群。结论"HIV抗体不确定"的产生可能与病例感染HCV、TP、HTLV-I/II,病例体内ANA、AFP水平升高有关。
Objective To examine the possible biological factors contributing to the observed uncertainty on the measurement of HIV antibody by Western blot. Methods Clinical samples from hepatitis B （HBV）, hepatitis C （HCV）, treponema （TP） and human T cell leukemia virus I/II （HTLV-I/II） infected patients showing uncertain HIV antibody by Western blot were checked for the serum level of carcino-embryonic antigen （CEA）, antinuclear antibody （ANA）, a-fetoprotein（AFP） and β2-microglobulin （β2-MG）. Serum from normal subjects was used as control. Results The rates of sero-positive for HCV, HTLVI/II and TP in these clinical samples were higher than the normal serum. 22.22% of these clinical samples had the level of ANA higher than the normal serum. 9.27% serum samples from non-pregnant women were higher than the normal subjects. Conclusion The observed uncertainty in the determination of HIV antibody is correlated with the patients with HCV, HTLV-I/II and/or TP infection. It is also correlated with the elevated level of serum ANA and/or AFP.
出处
《热带医学杂志》
CAS
2009年第9期1073-1075,共3页
Journal of Tropical Medicine
基金
广州市卫生局基金(No.2008-YB-123)
