摘要
目的:根据5种细胞系对脊髓灰质炎病毒1型(PV1)5个毒株的敏感性筛选适用于病毒消毒实验的病毒株和细胞系.方法:比较各毒株在不同细胞系中增殖3d~7d后的细胞病变作用(CPE)、半数组织培养感染里(TCⅡ)50)、蚀斑计数(PFU)及免疫印迹(DIBA)试验结果.结果:CPE与TCID)检测表明,同一细胞系Hep2和Hela中病毒感染细胞作用强弱为Henan,Hebei>Brunhide,Mahony>Sabin株;不同细胞系对同一病毒株的敏感性为Hep-2>BGM>Hela,Vero>RD细胞.DBA检测提示,Henan株较Brun-hide更易检出.结论:Henan株及Hep-2细胞可作为PV消毒实验所用的病毒和细胞.
Aim: To select suitable virus strains and celllines for experiment of inactivation of Poliovirus in water.Metheds: Comparing the results of cytopathic effects (CPE), 50% tissue culture infective doses (TCID50 ), plaqueforming units (PFU) and dot immunobinding assays(DIBA) after 5 virus strains were separately propagated for3~5 days in 5 cell lines. Results: Determination of CPEand TCID 50 showed that ① the virulence of 5 strains to thesame cell line ranged from the highest to the lowest asHenan, Hebei >Brunhide, Mahony> Sabin; ② Thesensitivities of 5 infected cell lines to the same strain were thefollowing range: Hep-2>BGM>Hela, Vero>RD.Detection of PFU and DIBA indicated that Henan strain was inspecied more easily than Brunhide strain after both strainswere propagated in Hep-2 cells. Conclusion: PV 1 Henanstrain and Hep-2 cell line are appropriate to the edperimentof inactivation of PV in water.
出处
《第四军医大学学报》
1998年第6期643-645,共3页
Journal of the Fourth Military Medical University