摘要
目的从致惊厥的行为学、安全性角度探讨咪哒唑仑对罗哌卡因致惊厥作用的影响。方法皮下注射咪哒唑仑20 min后,腹腔注射罗哌卡因,观察小鼠惊厥潜伏期、持续时间、未惊厥率及其死亡率:并按序贯法分析咪哒唑仑对罗哌卡因致惊厥半数有效量(ED_(50))和半数致死量(LD_(50))的影响。结果0.5、1.0 mg/kg咪哒唑仑可显著延长罗哌卡因致小鼠惊厥潜伏期(P<0.05,P<0.01),显著缩短惊厥持续期(P<0.01),减少惊厥发生率和死亡率;且本试验剂量咪哒唑仑(0.2、1.0 mg/mL与5.0、7.5 mg/mL)可分别增大罗哌卡因致惊厥ED_(50)和LD_(50)。结论咪哒唑仑可缓解罗哌卡因的致惊厥作用,结果对临床联合用药有一定的参考意义。
Objective To observe the influences of midazolam on ropivacaine-induced convulsion. Methods Midazolam was administered hypodermically, and twenty minutes later, ropivacaine was administered intraperitoneally. The convulsion latency, convulsion duration, incidence of no convulsion and mortality werer observed, and LD50 and the ED50 were analyzed by sequential method in mice. Results The convulsion latency of ropivacaine-induced convulsion were extended by midazolam, the convulsion duration were shorten, and the incidence of convulsion and mortality were reduction. The ED50 of ropivacaine-induced convulsion were improved by midazolam （0.2, 1.0 mg/kg） ; the LD50 were improved by midazolam （5.0, 7.5 mg/kg）. Conclusion Midazolam can antagonize the ropivacaine-induced convulsion.
出处
《世界临床药物》
CAS
2009年第10期606-608,619,共4页
World Clinical Drug
基金
国家自然科学基金资助项目(项目编号:30872432
30471657
39970715)
江苏省自然科学基金资助项目(项目编号:BK200143)
2009年江苏省高等学校大学生实践创新训练计划项目
关键词
咪哒唑仑
罗哌卡因
惊厥
半数有效量
半数致死量
midazolam
ropivacaine
convulsion
median effective dose
median lethal dose
