摘要
利用低能N^＋束注入技术对漆酶产生菌灵芝菌（Ganoderma lucidum）U60菌丝体进行辐照诱变。通过研究15keV能STUN注入剂量与灵芝菌存活率及突变率的生物学效应关系，确定了在2．6×10^15-3．9×10^15ions／cm^2注入剂量范围内可获得高比例正突变株。选择3．12×10^15ions／cm^2的注入剂量参数后，经多轮注入诱变，获得了遗传性稳定的漆酶高产突变株UIM-281；发酵产酶实验表明，UIM-281的产漆酶活力峰值分别是出发菌株U60的1．7倍及2．28倍，且产酶发酵周期相对缩短24h，是工业发酵中更加经济高效的灵芝漆酶发酵菌株。
The mycelium of Ganoderma lucidum U60 was implanted by Low-energy N^＋ ion beam technique to obtain the laccase high-yield strains. The dose of implanted ion with the higher proportion of positive mutation proportion was confirmed from 2.6 × 10^15 to 3.9 × 10^15 ions/cm^2 by investigating the relationship between the implantation dose under 15 keV energy and the suvival rates and the mutation rates of Ganoderma lucidum. The implanted ion dose of 3.12× 10^15 ions/cm^2 was choosed for the next step of multiple screening, and finally a laccase high-yield mutant UIM-281 with stable genetic character was obtained. The laccase-producing fermentation characteristics indicated that the peak values of laccase activity in the mutant UIM-281 was 1.7-fold and 2.28-fold higher than that of the original strain U60, moreover the fermentation time of the mutant was shortened as 24 h. These results suggest the mutant UIM-281 is the efficient and economic laccase-yielding Ganoderma lucidum strain in industrial fermentation.
出处
《激光生物学报》
CAS
CSCD
2009年第4期534-538,457,共6页
Acta Laser Biology Sinica
基金
安徽工程科技学院引进人才科研启动基金(2007YQQ007)
关键词
灵芝菌
低能离子束
漆酶
选育
Ganoderma lucidum
low-energy ion beam
laccase
screening
