摘要
从杭州炼油厂的土壤中筛选到的能降解二苯并噻吩（Dibenzothiophene,DBT）的菌株,并进行了显微形态、生理生化分析和16S rDNA序列分析,将其鉴定为Agrobacteriumtume faciens W3;对该菌株的降解产物分析表明,其降解DBT的终产物为2-羟基联苯（2-hydroxybiphenyl,2-HBP）,符合4-S脱硫途径;对该菌株的部分脱硫特性进行分析表明,5 g/L葡萄糖,3 g/L NH4Cl,0.15 mmol/L DBT,初始pH 6.9-7.5为其适宜的培养条件,培养时间72-96 h可达到最大生长,脱硫效果可达83%.
A dibenzothiophene （DBT）-degrading bacterium was isolated from oil-contaminated soil. It was identified as Agrobacterium tumefaciens W3 by physiological , biochemical test and 16S rDNA sequence analysis. The analysis of the metabolic product of DBT showed that the degrading mechanism of Agrobacterium tumefaciens W3 was 4-S mechanism. The cultivation and reaction conditions of Agrobacterium tumefaciens W3 were the Glucose concentration of 5 g/L, the NH4Cl concentration of 3 g/L, the DBT concentration of 0.15 mmol/L and initial pH 6.9 -7. 5 in 72-96 h. 83% of DBT was degraded.
出处
《浙江工业大学学报》
CAS
北大核心
2009年第5期520-524,共5页
Journal of Zhejiang University of Technology
基金
浙江省教育厅基金资助项目(D1055250)
关键词
生物脱硫
二苯并噻吩
根癌土壤杆菌W3
biodesulfurization catalyst
dibenzothiophene
Agrobacterium tumefaciens W3
