摘要
目的观察转换酶抑制剂（ＡＣＥＩ）苯那普利拉（Ｂｅｎａｚｅｐｒｉｌａｔ）对自发性高血压大鼠（ＳＨＲ）肾小球系膜细胞增殖的影响．方法采用细胞培养和３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）参入技术．结果（１）血管紧张素Ⅱ（ＡｎｇⅡ）组的３Ｈ－ＴｄＲ参入率高于对照组；（２）ＡｎｇⅡ加Ｂｅｎａｚｅｐｒｉｌａｔ组的３Ｈ－ＴｄＲ参入率低于ＡｎｇⅡ组；（３）ＡｎｇⅡ对ＳＨＲ肾小球系膜细胞增殖的刺激作用和Ｂｅｎａｚｅｐｒｉｌａｔ的抑制作用呈剂量依赖关系．结论苯那普利拉能逆转ＡｎｇⅡ对ＳＨＲ肾小球系膜细胞的增殖作用，从细胞水平体现其肾脏保护作用．
Objective\ To study the effect of benazeprilat on the proliferation of cultured glomerular mesangial cells of spontaneously hypertensive rats(SHR).\ Methods\ The proliferation of glumerular cell was detected by measuring 3H thymidine( 3H TdR) incorporation.\ Results\ 1. Compared with control group(fetal calf serum free medium, FCS free medium), the 3H TdR incorporation was high in angiotensin Ⅱ group(FCS free medium plus angiotensinⅡ,AngⅡgroup) ; 2. The 3H TdR incorporation of the AngⅡ plus benazeprilat group (free FCS medium plus Ang Ⅱ and benazeprilat) was lower than that of the AngⅡ group; 3. The stimulative effect of Ang Ⅱ and the inhibitory effect of benazeprilat of proliferation in glomerular mesangial cells of SHR were both dose dependent.\ Conclusion\ Benazeprilat may play an inhibitory role in the proliferation induced by Ang Ⅱ in glomerular mesangial cells of SHR. Benazeprilat has a renal protective effect at cellular level.
出处
《高血压杂志》
CSCD
1998年第4期295-297,共3页
Chinese Journal of Hypertension
关键词
苯那普利拉
高血压
系膜细胞
增殖
Benazeprilat
\ AngiotensinⅡ
\ Spontaneously hypertensive rat
\ Mesangial cell
\ Proliferation
