摘要
芥子酶在植物细胞中有可溶性的自由二聚体和不溶的结合形式两种存在形态.本研究发现物种间芥子酶的形态有较大差异.番木瓜芥子酶在种子、子叶和真叶等3种组织中,主要以可溶形式存在,不溶态芥子酶微量.而在油菜种子中,芥子酶主要以结合形式存在,在子叶和真叶中,芥子酶则主要以可溶形式存在.萝卜的芥子酶介于二者之间.供试3个物种的可溶性和不溶性芥子酶都存在相互抑制的现象.
Myrosinase was widely distributed in the crucifers. They catalize the degradation of glucosinolates and produce a series of products that provide a defense against herbivory. Plant myrosinases occur as free soluble dimmers or are bound in complexes with some other proteins（MBP and MyAP）,which is diverged among plant species and tissues or developmental stages. Myrosinase was extracted from three tissues of papaya, turnip and rape, both soluble and insoluble myrosinase activity were analyzed in a comparative way. Results indicated that papaya myrosinase was mainly presented in free form. The insoluble form of myrosinase presented low activity in three tested organs, including seeds, cotyledons and leaves. In contrast, insoluble form of myrosianse was the major myrosinase status in seeds of rape, although the soluble form in rape seeds were also high. Negative interaction between soluble form and insoluble form of myrosinases were observed.
出处
《湖北大学学报（自然科学版）》
CAS
北大核心
2009年第3期307-311,共5页
Journal of Hubei University：Natural Science
基金
国家自然科学基金(30571064)
海南省自然科学基金(80669)资助
关键词
番木瓜
芥子酶
硫代葡糖苷
活性浓度
Carica papaya L.
myrosinase
glucosinolate
activity concentration
