期刊文献+

猪囊尾蚴病患者外周血T细胞亚群比例和产生IFN-γ、IL-4水平研究 被引量:2

Study on the proportion of peripheval blood T cell subsets and the level of IFN-γ and IL-4 production in patients with cysticercosis
下载PDF
导出
摘要 目的:检测猪囊尾蚴病患者外周血T细胞(peripheral blood mononuclear cell,PBMC)体外诱生IFN-γ、IL-4的水平,探讨T细胞亚群在感染免疫中的调控。方法:采用流式细胞仪检测猪囊尾蚴病患者PBMC中T细胞亚群比例和IFN-γ/IL-4水平;用Cellquest软件或WinMDI2.8软件数据分析。结果:猪囊尾蚴病患者T细胞亚群CD3+与CD4+细胞百分率均较正常人升高(P<0.01),CD4+/CD8+比值亦较正常人升高(P<0.01);PBMC中分泌IFN-γ及IL-4的CD3+细胞百分率与正常人差异均无统计学意义(P>0.05)。结论:猪囊尾蚴病患者PBMC中CD3+、CD4+细胞百分率显著升高,T细胞亚群比例失调,免疫功能紊乱。 Objective:To detect the proportion of peripheral blood T cell subsets(PBMCs) and the level of IFN-γ/IL-4 production of peripheral blood in patients with cysticercosis, and to investigate the role of T cell subsets in immune regulation of cysticercus cellulosae infection. Methods:The proportions of T-cell subsets of PBMCs and Th1/Th2 cytokines producing cells in patients with cysticercosis were detected by four-color flow cytometry. Cellquest or WinMDI 2.8 was used in analysis of T-cell subsets. Results: The percentage of CD3^+ and CD4^+ T-cells subsets ,and the ratio of CD4^+/CD8 in patients with cysticercosis were higher than those of the control( P 〈 0.01 ). The percentage of secreting IFN-γ/IL-4 CD3 ^+ of PBMC in patients with cysticercosis was not higher than that of the control( P 〉 0.05 ). Conclusions: The patients with cysticercosis have the higher percentages of CD3^+ and CD4 ^+ cells than the control, presenting an imbalance of T cell subsets and abnormality of immunity.
出处 《蚌埠医学院学报》 CAS 2009年第9期756-758,共3页 Journal of Bengbu Medical College
基金 安徽省高校省级自然科学研究资助项目(kj2007B368ZC)
关键词 猪囊尾蚴病 流式细胞术 TH1/TH2细胞因子 cysticercosis flow cytometry Th1/Th2 cytokine
  • 引文网络
  • 相关文献

参考文献11

二级参考文献50

共引文献27

同被引文献26

引证文献2

二级引证文献8

;
使用帮助 返回顶部