摘要
目的:检测猪囊尾蚴病患者外周血T细胞(peripheral blood mononuclear cell,PBMC)体外诱生IFN-γ、IL-4的水平,探讨T细胞亚群在感染免疫中的调控。方法:采用流式细胞仪检测猪囊尾蚴病患者PBMC中T细胞亚群比例和IFN-γ/IL-4水平;用Cellquest软件或WinMDI2.8软件数据分析。结果:猪囊尾蚴病患者T细胞亚群CD3+与CD4+细胞百分率均较正常人升高(P<0.01),CD4+/CD8+比值亦较正常人升高(P<0.01);PBMC中分泌IFN-γ及IL-4的CD3+细胞百分率与正常人差异均无统计学意义(P>0.05)。结论:猪囊尾蚴病患者PBMC中CD3+、CD4+细胞百分率显著升高,T细胞亚群比例失调,免疫功能紊乱。
Objective:To detect the proportion of peripheral blood T cell subsets(PBMCs) and the level of IFN-γ/IL-4 production of peripheral blood in patients with cysticercosis, and to investigate the role of T cell subsets in immune regulation of cysticercus cellulosae infection. Methods:The proportions of T-cell subsets of PBMCs and Th1/Th2 cytokines producing cells in patients with cysticercosis were detected by four-color flow cytometry. Cellquest or WinMDI 2.8 was used in analysis of T-cell subsets. Results: The percentage of CD3^+ and CD4^+ T-cells subsets ,and the ratio of CD4^+/CD8 in patients with cysticercosis were higher than those of the control( P 〈 0.01 ). The percentage of secreting IFN-γ/IL-4 CD3 ^+ of PBMC in patients with cysticercosis was not higher than that of the control( P 〉 0.05 ). Conclusions: The patients with cysticercosis have the higher percentages of CD3^+ and CD4 ^+ cells than the control, presenting an imbalance of T cell subsets and abnormality of immunity.
出处
《蚌埠医学院学报》
CAS
2009年第9期756-758,共3页
Journal of Bengbu Medical College
基金
安徽省高校省级自然科学研究资助项目(kj2007B368ZC)