摘要
利用从葡萄中克隆到的白藜芦醇合酶基因(Resveratrol Synthase,简称RS)构建了含有35S组成型启动子的植物表达载体P35s-2300-RS,应用农杆菌介导方法对马铃薯品种进行遗传转化。通过对抗性芽、生根、再生植株的筛选,得到5株再生小苗。经PCR、Southern检测证实,有3株为真正的转基因植株。
The resveratrol synthase gene expression vector has been constructed with the construction of constitutive 35S prorooter of die plant expression vector P35s-2300-RS （Resveratrol Symhase,referred to as RS） ,The gene was originally cloned from grapes. The gene was transferred by the Agrobacterium-mediated into the potato varieties. The study obtained 5 regenerated seedlings by screening the resistant shoot.root,the regenerated plant,3 of which were the real transgenic plant confirmed by PCR,Southern Testing.
出处
《黑龙江农业科学》
2009年第5期1-4,共4页
Heilongjiang Agricultural Sciences
基金
黑龙江省留学回国人员科技活动项目(黑人函(2005)307)
黑龙江省博士后资助项目(LSZH-04086)
关键词
白藜芦醇合酶
马铃薯
表达载体
农杆菌介导
遗传转化
resveratrol synthase
potato
expression vector
agrobacterium-mediated
genetic transformation
