摘要
目的比较犬内皮祖细胞内衬脱细胞带瓣膜静脉支架与犬天然静脉瓣膜在生理条件下的闭合机制。方法普通雄性杂种犬36只,体重15～18kg,取12只犬左侧带瓣膜股静脉制备脱细胞带瓣膜静脉支架。将余24只动物随机分为实验组和对照组(n=12),取实验组骨髓进行体外扩增培养获取内皮祖细胞,取第3代细胞以5×106个/mL密度种植于支架上,行种植细胞前后支架大体及HE染色观察。实验组于左侧股静脉部位移植同种异体内皮祖细胞内衬脱细胞带瓣膜静脉支架,对照组原位移植自体带瓣膜静脉。术后4周通过彩色超声多普勒检查,比较动物由平卧位升至头高脚低位,瓣膜关闭前后瓣膜远端血流方向和流速的改变,以及瓣窦部位静脉管径的变化情况。结果种植细胞前后支架大体及HE染色示,经脱细胞处理的带瓣膜静脉支架能较好地保留其纤维和胶原结构,经生物反应器可于体外成功将内皮祖细胞种植于脱细胞支架上。动物于头高脚低位至瓣膜开始关闭时,实验组均出现血液返流现象,返流流速(1.4±0.3)cm/s;对照组未出现血液返流现象,但血液流速下降,由高峰流速(21.3±2.1)cm/s减至(18.2±3.3)cm/s。对照组瓣膜的活动周期、开始闭合时间及瓣膜从开始闭合到完全闭合的时间分别为(918±46)、(712±48)、(154±29)ms,实验组分别为(989±53)、(785±43)、(223±29)ms,两组比较差异均有统计学意义(P<0.05)。瓣膜关闭完全后,两组均未见返流。实验组和对照组瓣膜关闭前后瓣膜窦部静脉管径均有所增大,增大比例分别为116.8%±2.0%和118.5%±2.2%(P>0.05)。结论犬内皮祖细胞内衬脱细胞带瓣膜静脉支架与天然静脉瓣膜在生理条件下的闭合条件有明显差异,前者依赖于血液返流,后者与血液流速减慢及瓣窦段静脉压力增高有关。
Objective To compare canine decelluarized venous valve stent combining endothelial progenitor cells （EPC） with native venous valve in terms of venous valve closure mechanism in normal physiological conditions. Methods Thirty-six male hybrid dogs weighing 15-18 kg were used. The left femoral vein with valve from 12 dogs was harvested to prepare decelluarized valved venous stent combined with EPC. The rest 24 dogs were randomly divided into the experimental group and the control group （n=12 per group）. In the experimental group,EPC obtained from the bone marrow through in vitro amplification were cultured,the cells at passage 3 （5 × 10^6 cells/mL） were seeded on the stent,and the general and HE staining observations were performed before and after the seeding of the cells. In the experimental group,allogenic decelluarized valved venous stent combined with EPC was transplanted to the left femoral vein region,while in the control group,the autogenous vein venous valve was implanted in situ. Color Doppler Ultrasound exam was performed 4 weeks after transplantation to compare the direction and velocity of blood flow in the distal and proximal end of the valve,and the changes of vein diameter in the valve sinus before and after the closure of venous valve when the dogs changed from supine position to reverse trendelenburg position. Results General and HE staining observations before and after cell seeding：the decelluarized valved venous stent maintained its fi ber and collagen structure,and the EPC were planted on the decelluarized stent successfully through bioreactor. During the period from the reverse trendelenburg position to the starting point for the closure of the valve,the reverse flow of blood occurred in the experimental group with the velocity of （1.4 ± 0.3） cm/s; while in the control group,there was no reverse flow of blood,but the peak flow rate was decreased from （21.3 ± 2.1） cm/s to （18.2 ± 3.3） cm/s. In the control group,the active period of valve,the starting point for the
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2009年第10期1260-1263,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
组织工程血管
脱细胞静脉支架
静脉瓣膜
内皮祖细胞
彩色超声多普勒
犬
Tissue engineered vessel Decelluarized venous value stent Venous valve Endothelial progenitor cells Color Doppler Ultrasound Canine
