摘要
为了检测产志贺毒素大肠杆菌(STEC)在鸭源大肠杆菌中的分布情况,本研究建立检测STEC的多重PCR方法,针对STEC特有的毒力基因stx1、stx2、hlyA和eaeA筛选了4对引物,通过对PCR反应条件的优化、特异性和灵敏度的检测,建立检测STEC的多重PCR,并应用该方法调查254株鸭源致病性E.coli和115株外表健康鸭的泄殖腔分离的E.coli中STEC的分布情况。在254株鸭源致病性E.coli中检测出6株STEC,从外表健康鸭的泄殖腔分离的115株E.coli中未检测到STEC。检出的STEC的血清型分别为O36、O60、O77、O78、O158和O7&O92。本实验建立的检测STEC的多重PCR方法特异性好、灵敏度高;对鸭源E.coli的检测结果证实鸭致病性大肠杆菌中存在STEC菌株,分布频率较低,但其血清型具有广泛的宿主源,存在引起人类疾病的可能性。
A multiplex PCR was developed for rapid detection of the Shiga-Toxin producing Escherichia coli （STEC） using primers target to STEC-specific virulence genes stxl, stx2, hlyA and eaeA. The PCR assay was used to test 369 duck-origin E.coli including 254 pathogenic isolates and 115 isolates from healthy ducks. Six STEC strains were detected from 254 pathogenic E.coli, and none was found in 115 E.coli isolated from healthy ducks. The serotypes of STEC were identified as 036, 060, 077, 078, O158 and 078 ＆ 092. The results proved that STEC existed in ducks, but with a low distribution frequency. Due to the wide-spectrum hosts of STEC serotypes determined, it is inferred that duck-origin STEC may pose threat to the human health.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2009年第10期780-784,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
"十一五"国家科技支撑计划重大项目(2006BAD06A11)
国家菌种资源平台建设项目(2005DKA21205-11)
