摘要
目的探讨血红素氧合酶－1（hemeoxygenase1，HO－1）在胃腺癌及腹膜转移灶、细胞系及耐药细胞系中的表达及意义。方法用免疫组化法检测68例胃腺癌组织及其腹膜转移灶、转移灶旁无瘤腹膜组织中HO1的表达，以及46例无腹膜转移的胃腺癌组织中HO－1的表达。Western印迹法检测胃腺癌腹膜转移组织及耐药细胞系HO－1的表达。结果胃腺癌及其腹膜转移灶HO－1的阳性表达率分别为39．7％（27／68）和41．2％（28／68），显著高于癌旁无瘤腹膜组织[0％（0／68），P〈0．01]，亦显著高于无腹膜转移胃癌组织[21．7％（10／46），P〈0．05]。低分化转移灶HO—1表达水平显著高于中、高分化转移灶（P〈0．05）。Western印迹法检测胃癌腹膜转移患者的转移灶H01表达水平显著高于其癌旁无瘤腹膜组织（P〈0．05）。H01在耐药细胞系GC9811P的表达水平较其亲本细胞系明显上调（P〈0．06）。结论HO－1在胃腺癌腹膜转移过程中表达增高，HO－1可能参与胃癌腹膜转移发生。高表达HO－1与胃癌腹膜转移组织的恶性程度有关，其信号转导通路可能存在于组织的上皮细胞，并可能与多药耐药有关。
Objective To investigate the expression and significance of heme oxygenase-l（HO- 1 ） in gastric adenocarcinoma and its peritoneal metastatic tissues, as well as drug-resistant cell strains. Methods The expression of HO-1 in patients with （n=68） or without （n=46） peritoneal metastasis of gastric adenocarinoma was examined . The expression of HO-1 in cancerous tissue, peritoneal metastatic foci, and normal peritoneum was detected by immunohistochemistry. The expression of HO-1 protein in metastatic foci and drug-resistant cell strains was measured by Western blotting. Results The positive expression of HO-1 was 39.7% （27/68） in gastric adenocarcinoma tissues with metastasis and 41. 2% （28/68） in peritoneal metastatic tissues, which was significantly higher than that in normal peritoneum （0%,0/68 ,P〈0.01） and gastric adenocarcinoma tissues without metastasis （21.7%, 10/46 ,P〈0.05）. The Western blot study showed that the HO-1 expression in metastatic tissues was higher than that in normal peritoneum （P〈0.05）. The expression of HO-1 protein was markedly increased in GC9811 P drug-resistant cell strains compared with its parental cell strains （P 〈 0.05）. Conclusions The increased expression of HO-1 may be involved in the peritoneal metastasis of gastric adenocarcinoma, and related to the malignant potential. The underlying signal pathways in neopastic epithelium may also be related to the multi-drug resistance.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2009年第9期521-524,共4页
Chinese Journal of Digestion
基金
国家自然科学基金资助项目（30860332,30670969）
教育部“春晖计划”（Z2006-1-75001）
关键词
胃肿瘤
肿瘤转移
血红素氧合酶-1
多药耐药
Stomach neoplasms
Neoplasms metastasis
Hemeoxygenase-1
Drug resistance, multiple
