摘要
目的：细胞凋亡是细胞死亡的一种形式，对缺血后梗塞灶的发生、发展有一定的作用。故测定缺血脑区出现ＤＮＡ片断化的凋亡细胞数量变化及在缺血脑区的分布状况，以了解脑缺血的病理生理及寻求防治措施。方法：利用线栓法制成大脑中动脉缺血动物模型，测定脑组织ＳＯＤ、ＬＰＯ含量变化及凋亡细胞的密度变化；利用脱氧核苷酸末端转移酶介导的ｄＵＴＰ－荧光素缺口末端标记技术（ＴＵＮＥＬ）原位检测细胞核内的特征性ＤＮＡ片断，具特异性，在光镜下即可被辨认。结果：随缺血时间的持续，缺血侧凋亡细胞的密度增加，脑组织ＳＯＤ含量下降、ＬＰＯ上升；同一缺血时刻下视前区与纹状体的细胞凋亡密度高于皮层，即凋亡主要发生在缺血中心区近中线侧区域，提示“半暗带”的细胞死亡可能以凋亡形式为主，也可能是缺血后自由基介导的凋亡增加。结论：“半暗带”内细胞以凋亡形式死亡为主，对缺血灶不可逆性改变有重要作用。
Purpose:Apoptosis describes a specific form of cell death,and may be play an important role in development of this ischemic.Methods:The objective of this present study is to make animal brain ischemic model with middle cerebral artery occlusion(MCAO)by nylon thread,then measure the numbers and anatomic distribution of nerve cells apoptosis, assay the SOD and LPO content in same ischemic tissue. Results:The apoptic of nerve cells were primarily locatized to the inner boundary zone of the ischemic infarction,were distributed in penumbra zone, but in the central portion of the lesion was the necrosis,SOD content was decreased and LPO content was markedly increased.Conclusion:The results indicated that the ability of brain tissue to clean free radical was depressed in the ischemic area, so apoptosis is major form of cell death in penumbra zone,may contribute to the development of infarction after MCAO.This suggests to inhibite the free radical would be more important for controlling the ischemic tissue after strok.
出处
《卒中与神经疾病》
1998年第3期116-119,共4页
Stroke and Nervous Diseases
