摘要
该文研制了植物激素脱落酸(ABA)间接竞争酶联免疫试剂盒,该试剂盒可对ABA进行快速检测。文中成功制备了脱落酸和牛血清蛋白的交联物(ABA-BSA),并对交联物的浓度、抗体稀释度、酶标二抗稀释度对实验的影响进行了考察。结果表明,该试剂盒具有高灵敏性,最低检出浓度为1.38ng/mL,在10ng/mL～1000ng/mL之间,吸光度与浓度的对数呈良好的线性关系。样品添加回收率在95.98%～102.25%之间,平均回收率为100.18%,变异系数(CV)在1.27%～4.68%之间,平均变异系数为3.18%。
ABA was coupled to carrier protein bovine serum albumin（BSA）, and an indirect competitive enzymelinked immunosorbent assey （ciELASA） was developed for the quantitative determination to ABA. The concentration of ABA-BSA of coating ELISA plates, antibody dilution and enzyme labeled antibody dilution were optimized ,The results showed the working range was assigned to concentrations for 1 ng/mL～1 000 ng/mL ,with the detect ion limit being 1.38 ng/mL,The linear range of the assay was between10 ng/mL～1 000 ng/mL. The ELISA-kit was sta- ble ,simple, rapid, sensitive and can meet the demand of practical work.The recovery rate of fortified sample was between 95.98% - 102.25%, and mean recovery rate was 100.18%.The Coefficient of variance was between 1.27% - 4.68%, and mean coefficient of variance of intra- assays was 3.18%.
出处
《化学传感器》
CAS
2009年第3期29-33,共5页
Chemical Sensors
