摘要
目的清胰汤治疗急性胰腺炎(AP)尤其是并发内毒素血症的AP的效果及其可能的机制。方法Wistar大鼠40只,随机分为5组:A组(对照组),B组(AP组),C组(清胰汤处理的AP组),D组(伴有内毒素血症的AP组),E组(清胰汤处理的伴有内毒素血症的AP组);各组分别取胰腺组织行病理学检查并评分,用半定量RT-PCR检测TNF-αmRNA的表达水平,流式细胞术(FCM)检测NF-κB的激活。结果胰腺组织TNF-αmRNA表达水平,NF-κB激活及病理损伤AP组较对照组均明显升高,使用LPS可刺激NF-κB的激活并引起TNF-αmRNA表达的进一步升高及病理损伤的进一步加重,证实了NF-κB对TNF-α的调节作用及其与AP病理改变严重程度之间的关系;在使用了清胰汤干预后,可显著降低NF-κB激活及TNF-α的水平,同时也减轻了胰腺的病理损害。结论清胰汤通过下调NF-κB的活性及TNF-α的表达而发挥治疗AP及伴有内毒素血症AP的作用。
OBJECTIVE To study the effect of Qing Yi Tang on acute pancreatitis（AP）, especially AP complicated with endotoxemia and its possible mechanism. METHODS Fourty Wistar rats were divided into 5 groups including group A （the control group）, group B（the AP group）, group C （the AP group treated with Qing Yi Tang）, group D （the AP group treated with LPS） and group E （the AP group treated with LPS ＋ Qing Yi Tang）. Pathological damage of pancreatic tissue was scored with HE staining. The mRNA expression of TNF-α was measured with semi-quantitative RT-PCR, and activation of NF-κB was detected with flow cytometry（FCM） assay. RESULTS It was shown in results that the expression of TNF-α mRNA, activation of NF-κB and pathological damage of the group B were all obviously higher than those of the group A. After treated with LPS which might promote the activation of NF-κB, there was seen the further rise of the activation of NF-κB, expression of TNF-α mRNA and pathological damage. When Qing Yi Tang intervention was applied, the activation of NF-κB and the expression of TNF-α mRNA could be remarkably relieved, so did the pathological damage of pancreas. CONCLUSIONS Qing Yi Tang may be applied to decrease activation of NF-κB and the expression of TNF-α so as to treat AP or AP with endotoxemia.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第19期2539-2542,共4页
Chinese Journal of Nosocomiology
基金
黑龙江省卫生厅科研课题(2006-073)
黑龙江省十一五科技攻关计划资助项目(GB07C32505)
教育部新世纪优秀人材支持计划资助项目(NCET-07-0248)
关键词
急性胰腺炎
内毒素血症
炎症介质
Acute pancreatitis
Endotoxemia
Mediators of inflammation
