摘要
目的:建立家族性腺瘤性息肉病(FAP)家系永生细胞株,为FAP的深入研究提供永久的研究材料.方法:应用EB病毒(EBV)加入环孢霉素A转化技术,将FAP家系成员外周血B淋巴细胞转化成永生化淋巴母细胞系.结果:成功为两个FAP家系25个个体建立了永生细胞株,转化率为100%.对已建株保存的永生细胞进行复苏培养,复苏成功率为100%.结论:FAP永生细胞株保存了原来个体完整的基因组,为在分子水平上揭示FAP的发病机制提供了宝贵的材料.
AIM： To establish immortal lymphoblastoid cell lines of familial adenomatous polyposis （FAP） and to provide materials for further research of FAP. METHODS： Immortal lymphoblastoid cell lines of FAP were established by Epstein-Barr （EB） virus transformation of peripheral blood B lymphocytes from members of FAP and addition of cyclosporine A. RESULTS： Twenty-five immortal cell lines of two FAP pedigrees were successfully obtained with a transformation rate of 100%. The culture recovery rate was 100%. CONCLUSION： The established immortal lymphoblastoid cell lines are good materials used for molecular level study of FAP.
出处
《第四军医大学学报》
北大核心
2009年第18期1707-1709,共3页
Journal of the Fourth Military Medical University
基金
宁夏回族自治区教委资助(2005054)
