摘要
[目的]促进丝棉木种苗的快速繁殖并保持其优良性状。[方法]以丝棉木的嫩茎、叶片为材料,消毒后接种在不同激素水平的MS培养基上诱导产生幼苗。[结果]培养基MS+6-BA0.3mg/L+NAA0.10mg/L是丝棉木外植体诱导产生腋芽的最佳培养基。丝棉木叶片在培养基MS+6-BA0.3mg/L+NAA0.10mg/L+2,4-D0.05mg/L上能成功诱导出小苗,诱导出来的小苗叶色鲜绿、生长旺盛。在培养基MS+6-BA0.3mg/L+NAA0.10mg/L上幼苗的分化系数最高,且幼苗生长最好。在培养基1/2MS+IBA0.4mg/L+NAA0.10mg/L+根皮苷3mg/L上幼苗的生根率较高且根系较发达。在基质草炭+蛭石+珍珠岩(5:3:2)上移栽苗的生根时间最短,为10d,生根率和成活率最高,分别为95.6%和85.48%。[结论]该研究为丝棉木的组培育苗提供了技术支持。
[ Objective] The purpose of the research was to promote the rapid propagation of Euonymus bungeanus seedlings and keep their good traits. [ Method ] With the immature stems and leaves of E. bungeanus as materials, they were inoculated on MS media with different hormone levels to generate seedlings by induction after sterilization. [ Result] The medium MS ＋ 6-BA 0.3 mg/L ＋ NAA 0.10 mg/L was the optimum medium to generate auxiliary buds by induction from E. bungeanus explants. The seedlings could be induced from E. bungeanus leaves successfully on the medium MS ＋ 6-BA 0.3 mg/L ＋ NAA 0.10 mg/L ＋ 2,4-D 0.05 mg/L and the induced seedlings had brightly green leaves and grew vigorously. On the medium MS ＋6-BA 0.3 mg/L ＋ NAA 0.10 mg/L, the differentiation coefficient of seedlings was highest and the seedlings grew best. On the medium 1/2 MS ＋ IBA 0.4 mg/L ＋ NAA 0.10 mg/L ＋ phloridzin 3 mg/L, the rooting rate of seedlings was higher and their roots were more developed. On the substrate peat ＋ vermiculite ＋ perlite （5： 3： 2）, the rooting time of transplanted seedlings was shortest, being 10 d and their rooting rate and survival rate were highest, being 95.6% and 85.48% resp. [ Conclusion] The research supplied technical support for the tissue culture and raising seedling of E. bungeanus.
出处
《安徽农业科学》
CAS
北大核心
2009年第30期14625-14626,14652,共3页
Journal of Anhui Agricultural Sciences
基金
宁夏生态工程学校资助项目
关键词
丝棉木
外植体
嫩茎
叶片
组织培养
Euonymus bungeanus
Explants
Immature stem
Blade
Tissue culture
