摘要
采用热水法从大粒车前子中提取、分离出多糖(Plantago asiaticaL.crude polysaccharide,PLCP),加水复溶后依次用40%和60%乙醇分级,得到不同级分多糖(PLCP-1和PLCP-2),上清液部分记为PLCP-3。凝胶柱层析法考察PLCP-1、PLCP-2和PLCP-3凝胶色谱行为,GC测定单糖组成,红外和紫外光谱检测光谱性质,并测定糖含量、糖醛酸含量和蛋白含量。结果表明:PLCP-1、PLCP-2和PLCP-3均为酸性多糖,蛋白含量较低,其糖含量依次为71.06%、76.71%和64.78%。紫外和红外测定结果显示,PLCP-1、PLCP-2和PLCP-3均为酸性多糖,且含有一定量的蛋白。PLCP-1、PLCP-2和PLCP-3主要含有阿拉伯糖和木糖,还有少量的鼠李糖、葡萄糖、甘露糖和半乳糖等,但具体摩尔组成比例相差较大。
Crude polysaccharides extracted from seeds of Plantago asiatica L. （Plantago asiatica L. crude polysaccharide, PLCP） with hot water were successively fractionated by different concentrations （40% and 60%） of ethanol into two precipitation fractions named PLCP-1 and PLCP-2 and one supematant fraction name PLCP-3. The physicochemical properties of PLCP-1, PLCP-2 and PLCP-3 were analyzed by UV and IR spectroscopy, GC and Sephacryl TM S-400 HR gel-filtration chromatography. UV and IR spectral analysis indicated that PLCP-1, PLCP -2 and PLCP-3 all were acidic polysaccharides containing a little amount of protein. The sugar contents of PLCP-1, PLCP -2 and PLCP-3 were 71.06%, 76.71% and 64.78%, respectively. Arabinose and xylose were the main monosaccharides in the polysaccharides and other monosaccharides such as rhamnose, glucose, mannose and galactose were also detected but the tool ratios of the monosaccharides were different.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第17期34-37,共4页
Food Science
基金
国家自然科学基金项目(20802032)
高等学校博士学科点专项科研基金项目(200804030001)
江西省自然科学基金项目(2008GZH0050)
江西省教育厅2008年度科技计划项目(GJJ08056)
关键词
大粒车前子多糖
乙醇分级
凝胶色谱
单糖组成
光谱性质
polysaccharide from seeds of Plantago asiatica L.
ethanol fractionation
gel chromatography： monosaccha-fide composition
spectral properties
