摘要
背景:自主设计的可降解镁锌合金既保持了镁合金与人骨接近的密度和弹性模量,又排除了商业镁合金中铝和稀土元素的毒性。目的:观察新型可降解镁锌合金(Mg-6%Zn)对前成骨细胞MC3T3-E1细胞整合素β1表达的影响。设计、时间及地点:细胞分子学水平,对比观察实验,于2008-03/05在上海交通大学附属第六人民医院中心实验室完成。材料:实验可降解Mg-6%Zn合金由上海交通大学材料科学与工程学院研制,密度是1.82g/cm3,弹性模量44GPa,以临床常用的可吸收内植物左旋聚乳酸做为对照。MC3T3-E1细胞由中国科学院典型培养物保藏委员会细胞库提供。方法:将MC3T3-E1细胞接种于镁锌合金和左旋聚乳酸上,共培养2,24,48h后用扫描电镜观察细胞形态;共培养1,3,6,9,12,15d后,收集细胞,利用实时荧光定量PCR方法检测成骨细胞整合素β1mRNA的表达水平。主要观察指标:MC3T3-E1细胞在材料表面生长形态和整合素β1mRNA的表达水平。结果:在镁锌合金表面MC3T3-E1细胞黏附性能优于左旋聚乳酸表面;镁锌合金表面培养的MC3T3-E1细胞在实验期间能持续表达整合素β1mRNA,表达水平随着时间延长而增高(P<0.01),但与左旋聚乳酸相比差异无显著性意义(P>0.05)。结论:镁锌合金表面MC3T3-E1细胞黏附性能优于左旋聚乳酸表面,但并非通过诱导整合素β1的表达水平来介导的。
BACKGROUND： A novel biodegradable Mg-Zn alloy is designed which the density and the Young＇s modulus are proximal to human bone. At the same time, it depletes the toxicity of aluminium and rare earth element in commercial magnesium alloys. OBJECTIVE： To observe the effect of Mg-Zn alloy （Mg-6%Zn） on the integrin β1 expression of preosteoblasts MC3T3-E1. DESIGN, TIME AND SETTING： A contrast study was performed at the Central Laboratory of the Sixth People＇s Hospital Affiliated to Shanghai Jiao Tong University between March and May 2008. MATERIALS： The Mg-6% Zn was prepared by School of Materials Science and Engineering of Shanghai Jiao Tong University, which the density was 1.82 g/cm^3 and the Young＇s modulus was nearly 44 GPa. Poly-L-lactic acid （PLLA） was used as the controls. MC3T3-E1 cells were provided by Chinese Academy of Science Type Culture Collection. METHODS： The cell attachment was observed after cultured with Mg-Zn and PLLA at 2, 24 and 48 hours under scanning electron microscope; the integrin β1 mRNA expression of MC3T3-E1 cultured with Mg-Zn and PLLA was estimated by real-time fluorescent quantitative polymerase chain reaction （real-time PCR） at days 1, 3, 6, 9, 12 and 15 after culture. MAIN OUTCOME MEASURES： The MC3T3-E1 cells attachment on the material surface and the integrin β1 mRNA expression. RESULTS： MC3T3-E1 cell adhesion was better on the Mg-Zn alloy surfaces than on the PLLA surface; The integrin β1 mRNA of osteoblasts on Mg-Zn kept on expressing during experiment and increased with time （P 〈 0.01）, but there was no significantly difference between the two groups at the same time （P 〉 0.05）. CONCLUSION： MC3T3-E1 cell adhesion is better on the Mg-Zn alloy surfaces than on the PLLA surface, but it is not mediated by inducing the integrin β1 mRNA expression.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第38期7531-7534,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金项目资助(30772182)~~
