摘要
利用传统的水提醇沉法从玉米芯提取可溶性糖,通过部分酸水解、葡聚糖凝胶SephadexG-25柱层析、薄层层析(TLC)、高效液相色谱(HPLC)等对玉米芯中低聚木糖的组成及含量进行研究。结果表明:玉米芯中低聚木糖种类以木二糖和木三糖为主,提取纯化得木二糖的纯度可达95.63%,得率为6.84%;木三糖的含量则较低,仅为0.22%。
Water-soluble crude polysaccharides obtained from corncob through water extraction and ethanol precipitation were partially hydrolyzed by trifluoroacetic acid. The crude polysaccharides and their hydrolysates were separately purified （fractionated） by Sephadex G-25 chromatography to be fractions A and B and they were then analyzed by thin-layer chromatog- raphy （TLC） and high-pressure liquid chromatography （HPLC）. Xylobiose and xylotriose were major components of xylooligosaccharides from corncob. Fraction B mainly consisted of xylobiose with a yield of 6.84% in chromatographic purification and the xylobiose content was as high as 95.63%. However, the xylotriose content was only 0.22%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第19期125-127,共3页
Food Science
基金
吉林省教育厅基金资助项目(2007第415号)
关键词
玉米芯
低聚木糖
木二糖
木三糖
组成
含量
corncob
xylooligosaccharides
xylobiose
xylotriose
component
content
