摘要
【目的】分析西农萨能奶山羊不同微卫星基因位点的基因型和基因型组合在多胎性状形成中的贡献率,探索优良基因的遗传聚合效应。【方法】利用微卫星标记与群体系谱记录,以优秀多胎个体为研究起点,上溯亲代,跟踪子代,检测典型优秀母羊个体的基因型组合及其与产羔数的关系。【结果】在西农萨能奶山羊群体中找到A3A7、B1B6、C1C5和D5D94对产羔率具有正效应的标记,以及A1A5、B4B9、C2C6和D4D84对产羔率具有负效应的标记。基因型组合A3A7B1B6C1C5D5D9的聚合效应值最高(P<0.05);分析比较几个基因型对产羔数的聚合效应值可知,D5D9较D4D9高5.11%,A3A7较A1A6高15.32%,C1C5较C2C6高8.11%,B1B6较B5B10高8.50%,D7D10较D4D9高10.09%,D5D9较D1D5高15.62%,D4D9较D1D5高11.08%,D2D6较D1D5高39.64%。【结论】在亲代到F1代的基因传递过程中,基因型组合具有明显的聚合效应;在F1代到F2代的基因传递过程中,基因型组合出现了明显的分离现象。
[Objective] The aim of this experiment was to analyze the pyramiding effect of genotype and genotype combination of mierosatellite loci in the polyembryony trait of Xinong Saanen goat. [Method] The relationship of genotype combination and litter size were compared and revealed in excellent Xinong Saanen goat by micro-satelite marker and family trees. [Result] The results indicate that there are genetic polymorphisms at these four microsatellite loci, and there are four positive genotypes （A3A7, B1B6, C1C5 and D5D9 ） and negative ones （A1A5, B4B9, C2C6 and D4D8） effects on litter size, respectively in Xinong Saanen goat. The pyramiding effect of A3A7B1B6C1C5D5D9 genotype combination markedly improved （P〈0.05） compared with the others. The effect of D5D9 genotype on litter size was higher than that of D4D9 by 5.11%,and A3A7 higher than A1A6 by 15.32%,CIC5 higher than C2C6 by 8.11%B1B6 higher than B5B10 by 8.50% ,D7D10 higher than D4D9 by 10.09% ,D5D9 higher than D1D5 by 15.62%;D4D9 higher than D1D5 by 11.08 %, and D2D6 higher than D1D5 by 39.64%. [Conclusion] There was＇a significant pyramiding effect in the course of reproduction between parental generation and F1 generation;and there was a significant gene isolation effect in the course of reproduction between F1 and F2 generation.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第10期47-54,共8页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家"863"高技术研究与发展计划项目(2007AA10Z167)
国家"十一五"奶业重大科技支撑项目(2006BAD04A11)
