摘要
目的:探讨FAAH在正常结肠壁内表达及意义.方法:收集2006-2008年由于结肠造瘘和结肠息肉等在我院行结肠手术的患者16例正常结肠组织,其中男10例,女6例.取其结肠标本,其中升结肠5例,降结肠3例,乙状结肠8例,分别采用免疫组织化学、Western blot及RT-PCR检测FAAH在正常结肠组织中的表达,并通过免疫荧光法对比FAAH与CB1在正常结肠壁中的表达.结果:在升结肠、降结肠及乙状结肠肠壁肌间神经元胞质内均可见阳性染色,同时在结肠黏膜层内的肠腺体以及吸收细胞的胞质中也可见明显阳性染色,且染色强度在各组基本相同,统计学差异不显著(P>0.05).Western blot的结果与免疫组织化学一致,灰度分析结果显示统计学差异不显著(P>0.05).RT-PCR显示,各组样本中均可检测到不同数量的FAAH拷贝数,每微克RNA中FAAH cDNA的拷贝数相比,差异无统计学意义(P>0.05);免疫荧光法提示在肠壁肌间神经元胞质中红、绿色荧光均可见,两者位置相近,分布呈互补趋势.结论:正常结肠壁中存在FAAH的表达,且各段肠管之间无差异,在肌间神经丛内FAAH的分布与CB1受体呈对应趋势,通过对大麻酚信号通路研究可能为功能性胃肠病的治疗提供新的手段.
AIM： To explore the significance of expression of fatty acid amide hydrolase （FAAH） in normal colon wall. METHODS： Sixteen normal colon specimens were collected from the ascending colon （five cases）, descending colon （three cases） and sigmoid colon （eight cases） in patients treated at our hospital due to colon polyps or undergoing colostomy. The expression of FAAH mRNA and protein in these specimens was detected by RT-PCR, immunohistochemistry and Western blot, respectively. The expression patterns of FAAH and cannabinoid receptor 1 （CB1） were compared by immunofluorescent staining.RESULTS： Positive staining for FAAH was observed in the cytoplasm of myenteric neurons in the intestinal wall in different colonic segments. Obvious positive staining was also noted in mucosal glands and the cytoplasm of absorptive cells. Semi-quantitative analysis showed that there were no significant differences in the stain- ing intensity among different colonic segments （all P 〉0.05）. Similar result was obtained by Western blot （all P 〉 0.05）. Real-time PCR results showed that there were also no significant differences in FAAH mRNA expression levels among different colonic segments （all P 〉 0.05）. Immunofluorescence staining showed that both FAAH and CB1 could be detected in the cytoplasm of myenteric neurons in the intestinal walls, and they were distributed in a complementary pattern. CONCLUSION： FAAH is expressed in normal colon wall. There are no significant differences in the expression levels of FAAH among different colonic segments. FAAH and CB1 are distributed in a complementary pattern.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第22期2266-2271,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30700917~~
关键词
大麻酚信号通路
脂肪酰胺水解酶
G蛋白偶联受体
Cannabinoid signaling pathway
Fatty acid amide hydrolase
Cannabinoid receptor 1
